核糖体中依赖延伸因子I和延伸因子II的鸟苷三磷酸酶活性之间的关系。蓖麻毒素对这两种活性的抑制作用。
Relationship between elongation factor I- and elongation factor II- dependent guanosine triphosphatase activities of ribosomes. Inhibition of both activities by ricin.
作者信息
Sperti S, Montanaro L, Mattioli A, Testoni G
出版信息
Biochem J. 1975 Jun;148(3):447-51. doi: 10.1042/bj1480447.
Abstract
The elongation factor 1- and elongation factor 2-dependent GTPase (guanosine triphosphatase) activities of ribosomes are inhibited by ricin, a toxic protein known to inactivate the 60S ribosomal subunit. It is suggested that also in eukaryotic ribosomes a "GTPase site', located on the larger subunit, is common to the two elongation factors.
摘要
核糖体的延伸因子1和延伸因子2依赖性鸟苷三磷酸酶（GTPase）活性受到蓖麻毒素的抑制，蓖麻毒素是一种已知可使60S核糖体亚基失活的毒性蛋白。有人提出，在真核生物核糖体中，位于较大亚基上的一个“GTPase位点”是这两种延伸因子共有的。
相似文献
1
Relationship between elongation factor I- and elongation factor II- dependent guanosine triphosphatase activities of ribosomes. Inhibition of both activities by ricin.核糖体中依赖延伸因子I和延伸因子II的鸟苷三磷酸酶活性之间的关系。蓖麻毒素对这两种活性的抑制作用。
Biochem J. 1975 Jun;148(3):447-51. doi: 10.1042/bj1480447.
2
Correlation of inhibition of ribosomal GTPase and protein synthesis by abrin and ricin.相思子毒素和蓖麻毒素对核糖体GTP酶的抑制作用与蛋白质合成的相关性。
Biochem Biophys Res Commun. 1977 Apr 25;75(4):845-50. doi: 10.1016/0006-291x(77)91459-0.
3
On the mechanism of protein-synthesis inhibition by abrin and ricin. Inhibition of the GTP-hydrolysis site on the 60-S ribosomal subunit.关于相思子毒素和蓖麻毒素抑制蛋白质合成的机制。对60-S核糖体亚基上GTP水解位点的抑制作用。
Eur J Biochem. 1975 Nov 15;59(2):573-80. doi: 10.1111/j.1432-1033.1975.tb02484.x.
4
Effect of kirromycin on elongation factor Tu. Location of the catalytic center for ribosome-elongation-factor-Tu GTPase activity on the elongation factor.奇霉素对延伸因子Tu的作用。核糖体-延伸因子-Tu GTP酶活性催化中心在延伸因子上的定位。
Eur J Biochem. 1977 May 2;75(1):55-65. doi: 10.1111/j.1432-1033.1977.tb11503.x.
5
Characterization of the ribosomal properties required for formation of a GTPase active complex with the eukaryotic elongation factor 2.与真核生物延伸因子2形成GTP酶活性复合物所需核糖体特性的表征
Eur J Biochem. 1989 Feb 15;179(3):603-8. doi: 10.1111/j.1432-1033.1989.tb14589.x.
6
Inhibition of protein synthesis in vitro by crotins and ricin. Effect on the steps of peptide chain elongation.巴豆毒素和蓖麻毒素对体外蛋白质合成的抑制作用。对肽链延伸步骤的影响。
Biochem J. 1976 Apr 15;156(1):7-13. doi: 10.1042/bj1560007.
7
A ribosome-dependent GTPase from yeast distinct from elongation factor 2.一种来自酵母的核糖体依赖性GTP酶，与延伸因子2不同。
Proc Natl Acad Sci U S A. 1976 Jan;73(1):73-6. doi: 10.1073/pnas.73.1.73.
8
Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用：60S核糖体亚基作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):813-5. doi: 10.1042/bj1360813.
9
Activity of the 30-S CsCl core in elongation-factor-dependent GTP hydrolysis.30-S CsCl核心在依赖延伸因子的GTP水解中的活性。
Eur J Biochem. 1976 Jan 2;61(1):317-23. doi: 10.1111/j.1432-1033.1976.tb10024.x.
10
Structure and phosphorylation of an acidic protein from 60S ribosomes and its involvement in elongation factor-2 dependent GTP hydrolysis.来自60S核糖体的一种酸性蛋白的结构与磷酸化及其在延伸因子2依赖性GTP水解中的作用
Biochem Biophys Res Commun. 1977 Aug 8;77(3):989-98. doi: 10.1016/s0006-291x(77)80075-2.
引用本文的文献
1
LRP1 Shedding in Ricin-Induced Lung Injury: A Cell-Specific Response to Toxin Exposure.蓖麻毒素诱导的肺损伤中LRP1的脱落：对毒素暴露的细胞特异性反应。
Int J Mol Sci. 2025 Jun 6;26(12):5448. doi: 10.3390/ijms26125448.
2
Medical Countermeasures against Ricin Intoxication.抗蓖麻毒素医疗对策。
Toxins (Basel). 2023 Jan 20;15(2):100. doi: 10.3390/toxins15020100.
3
The low density receptor-related protein 1 plays a significant role in ricin-mediated intoxication of lung cells.低密度脂蛋白受体相关蛋白 1 在蓖麻毒素介导的肺细胞中毒中发挥重要作用。
Sci Rep. 2020 Jun 2;10(1):9007. doi: 10.1038/s41598-020-65982-2.
4
Ricin: An Ancient Story for a Timeless Plant Toxin.蓖麻毒素：古老的故事，永恒的植物毒素。
Toxins (Basel). 2019 Jun 6;11(6):324. doi: 10.3390/toxins11060324.
5
How Ricin Damages the Ribosome.蓖麻毒素如何损伤核糖体。
Toxins (Basel). 2019 Apr 27;11(5):241. doi: 10.3390/toxins11050241.
6
Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy.基于活性的数字全息显微镜的蓖麻毒素和相思豆毒素全细胞多参数检测法。
Toxins (Basel). 2019 Mar 22;11(3):174. doi: 10.3390/toxins11030174.
7
The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA.真菌核糖核酸酶样效应蛋白 CSEP0064/BEC1054 抑制植物免疫并干扰宿主核糖体 RNA 的降解。
PLoS Pathog. 2019 Mar 11;15(3):e1007620. doi: 10.1371/journal.ppat.1007620. eCollection 2019 Mar.
8
Ribosome-Inactivating Proteins from Plants: A Historical Overview.植物核糖体失活蛋白：历史概述
Molecules. 2016 Nov 26;21(12):1627. doi: 10.3390/molecules21121627.
9
Pokeweed antiviral protein, a ribosome inactivating protein: activity, inhibition and prospects.商陆抗病毒蛋白，一种核糖体失活蛋白：活性、抑制作用及前景
Toxins (Basel). 2015 Jan 28;7(2):274-98. doi: 10.3390/toxins7020274.
10
Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation.原子诱变揭示 23S 核糖体 RNA 的 A2660 是 EF-G GTP 酶激活的关键。
Nat Chem Biol. 2010 May;6(5):344-51. doi: 10.1038/nchembio.341. Epub 2010 Mar 28.
本文引用的文献
1
[Preparation and toxicity of pure ricin].[纯蓖麻毒素的制备与毒性]
Bull Soc Chim Biol (Paris). 1951;33(10):1461-6.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reaction.氨酰基转移反应中氨酰基-tRNA结合、肽键合成及转位酶活性的证据。
Arch Biochem Biophys. 1968 May;125(2):497-505. doi: 10.1016/0003-9861(68)90607-3.
4
Evidence for the enzymatic binding of aminoacyl transfer ribonucleic acid to rat liver ribosomes.氨酰基转移核糖核酸与大鼠肝脏核糖体酶促结合的证据。
J Biol Chem. 1968 Feb 25;243(4):791-8.
5
Interaction of polypeptide chain elongation factors with rat liver ribosomal subunits.多肽链延伸因子与大鼠肝脏核糖体亚基的相互作用。
J Mol Biol. 1969 Dec 28;46(3):447-57. doi: 10.1016/0022-2836(69)90188-0.
6
The sequence of reactions leading to the synthesis of a peptide bond on reticulocyte ribosomes.导致在网织红细胞核糖体上合成肽键的反应序列。
Cold Spring Harb Symp Quant Biol. 1969;34:331-45. doi: 10.1101/sqb.1969.034.01.040.
7
Studies on elongation factor II from calf brain.小牛脑延伸因子II的研究。
Arch Biochem Biophys. 1972 Sep;152(1):114-24. doi: 10.1016/0003-9861(72)90199-3.
8
Translation: its mechanism and control.翻译：其机制与控制。
Science. 1972 Apr 14;176(4031):195-7. doi: 10.1126/science.176.4031.195.
9
The mode of action of thiostreption in vivo.硫链丝菌素在体内的作用方式。
Biochem Biophys Res Commun. 1971 Aug 20;44(4):912-7. doi: 10.1016/0006-291x(71)90798-4.
10
Thiostrepton, an inhibitor of 5OS ribosome subunit function.硫链丝菌素，一种50S核糖体亚基功能抑制剂。
J Bacteriol. 1970 Mar;101(3):1073-5. doi: 10.1128/jb.101.3.1073-1075.1970.
Zotero 插件