Valles Steven M, Strong Charles A, Oi David H, Porter Sanford D, Pereira Roberto M, Vander Meer Robert K, Hashimoto Yoshifumi, Hooper-Bùi Linda M, Sánchez-Arroyo Hussein, Davis Tim, Karpakakunjaram Vedham, Vail Karen M, Fudd Graham L C, Briano Juan A, Calcaterra Luis A, Gilbert Lawrence E, Ward Rufina, Ward Kenneth, Oliver Jason B, Taniguchi Glenn, Thompson David C
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
J Invertebr Pathol. 2007 Sep;96(1):18-27. doi: 10.1016/j.jip.2007.02.006. Epub 2007 Feb 25.
Studies were conducted to examine the phenology, geographic distribution, and host specificity of the Solenopsis invicta virus-1 (SINV-1). Two genotypes examined, SINV-1 and -1A, exhibited similar seasonal prevalence patterns. Infection rates among colonies of S. invicta in Gainesville, Florida, were lowest from early winter (December) to early spring (April) increasing rapidly in late spring (May) and remaining high through August before declining again in the fall (September/October). Correlation analysis revealed a significant relationship between mean monthly temperature and SINV-1 (p<0.0005, r=0.82) and SINV-1A (p<0.0001, r=0.86) infection rates in S. invicta colonies. SINV-1 was widely distributed among S. invicta populations. The virus was detected in S. invicta from Argentina and from all U.S. states examined, with the exception of New Mexico. SINV-1 and -1A were also detected in other Solenopsis species. SINV-1 was detected in Solenopsis richteri and the S. invicta/richteri hybrid collected from northern Alabama and Solenopsis geminata from Florida. SINV-1A was detected in S. geminata and Solenopsis carolinensis in Florida and the S. invicta/richteri hybrid in Alabama. Of the 1989 arthropods collected from 6 pitfall trap experiments from Gainesville and Williston, Florida, none except S. invicta tested positive for SINV-1 or SINV-1A. SINV-1 did not appear to infect or replicate within Sf9 or Dm-2 cells in vitro. The number of SINV-1 genome copies did not significantly increase over the course of the experiment, nor were any cytopathic effects observed. Phylogenetic analyses of SINV-1/-1A nucleotide sequences indicated significant divergence between viruses collected from Argentina and the U.S.
开展了多项研究以考察红火蚁病毒 -1（SINV -1）的物候学、地理分布及宿主特异性。所检测的两种基因型，即SINV -1和 -1A，呈现出相似的季节性流行模式。在佛罗里达州盖恩斯维尔的红火蚁蚁群中，感染率从初冬（12月）至早春（4月）最低，在春末（5月）迅速上升，并在8月前一直保持高位，之后在秋季（9月/10月）再次下降。相关性分析显示，月平均温度与红火蚁蚁群中SINV -1（p<0.0005，r = 0.82）和SINV -1A（p<0.0001，r = 0.86）的感染率之间存在显著关系。SINV -1在红火蚁种群中广泛分布。该病毒在来自阿根廷的红火蚁以及除新墨西哥州外所检测的所有美国各州的红火蚁中均被检测到。在其他火蚁属物种中也检测到了SINV -1和 -1A。在从阿拉巴马州北部采集的黑火蚁以及火蚁属/黑火蚁杂交种和来自佛罗里达州的热带火蚁中检测到了SINV -1。在佛罗里达州的热带火蚁和卡罗来纳火蚁以及阿拉巴马州的火蚁属/黑火蚁杂交种中检测到了SINV -1A。在从佛罗里达州盖恩斯维尔和威利斯顿的6个陷阱诱捕实验中收集的1989只节肢动物中，除红火蚁外，没有其他物种检测出SINV -1或SINV -1A呈阳性。SINV -1在体外似乎不会感染Sf9或Dm -2细胞或在其中复制。在实验过程中，SINV -1基因组拷贝数没有显著增加，也未观察到任何细胞病变效应。对SINV -1/-1A核苷酸序列的系统发育分析表明，从阿根廷和美国采集的病毒之间存在显著差异。
