Henderson E
Department of Zoology and Genetics, Iowa State University, Ames 50011.
Nucleic Acids Res. 1992 Feb 11;20(3):445-7. doi: 10.1093/nar/20.3.445.
The atomic force microscope (AFM) was used to image supercoiled plasmid DNA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100-150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.
原子力显微镜（AFM）用于对沉积在云母表面处于水合或干燥状态的超螺旋质粒DNA进行成像。在仪器操作员确定的位置，通过扫描探针精确切割水合质粒。切除了小片段DNA（长度为100 - 150纳米），并将其沉积在切割后的质粒附近，这表明通过AFM可以从特定染色体位置去除和操纵光学显微镜无法分辨的基因组DNA片段。
