Tufan Hale, Zaki Basim M, Tecder-Unal Müge, Erdem S Remzi, Take Gülnur
Başkent University, Faculty of Medicine, Department of Pharmacology, Ankara, Turkey.
Ann Plast Surg. 2007 Apr;58(4):441-8. doi: 10.1097/01.sap.0000241682.42511.07.
The effect of captopril, angiotensin-converting enzyme inhibitor, on angiogenesis in several reports remained unclear. Its effect on neovascularization in rat abdominal skin flaps was investigated. Flap elevation, based on the right superficial inferior epigastric artery was performed with or without the administration of captopril (10 mg/kg/d), Ang II (100 microg/kg/d), or captopril and Ang II cotreatment. Mean arterial pressure (MAP), microangiography, capillary density measurement, necrosis area determination, laser Doppler flowmetry (LDF), AT1 and vascular endothelial growth factor (VEGF) immunostaining were used to evaluate the effects of captopril and the interaction between captopril and Ang II on the angiogenesis. Ang II and captopril cotreatment improved angiogenesis more than Ang II or captopril alone. The reduction of necrosis, enhancement of vascular network formation, capillary density, VEGF immunostaining, and local blood flow were evident in the cotreated group. We suggest that Ang II and captopril cotreatment improves ischemia-induced angiogenesis and increased viability and vascularity of skin flap in rats.
在一些报告中，血管紧张素转换酶抑制剂卡托普利对血管生成的影响仍不明确。本研究调查了其对大鼠腹部皮瓣新生血管形成的影响。以右侧腹壁浅动脉为蒂掀起皮瓣，同时给予或不给予卡托普利（10毫克/千克/天）、血管紧张素II（100微克/千克/天），或卡托普利与血管紧张素II联合治疗。采用平均动脉压（MAP）、微血管造影、毛细血管密度测量、坏死面积测定、激光多普勒血流仪（LDF）、AT1和血管内皮生长因子（VEGF）免疫染色来评估卡托普利的作用以及卡托普利与血管紧张素II之间的相互作用对血管生成的影响。血管紧张素II与卡托普利联合治疗比单独使用血管紧张素II或卡托普利更能促进血管生成。联合治疗组坏死减少、血管网络形成增强、毛细血管密度增加、VEGF免疫染色增强以及局部血流增加均很明显。我们认为，血管紧张素II与卡托普利联合治疗可改善缺血诱导的血管生成，并提高大鼠皮瓣的活力和血管化程度。
