Rachoń Dominik, Vortherms Tina, Seidlová-Wuttke Dana, Wuttke Wolfgang
Department of Clinical and Experimental Endocrinology, University of Göttingen, Göttingen, Germany.
Menopause. 2007 Sep-Oct;14(5):925-32. doi: 10.1097/GME.0b013e31802d979b.
To evaluate the effects of dietary equol, a metabolite of the phytoestrogen daidzein, on body weight gain, intra-abdominal fat accumulation, plasma leptin, lipids, and glucose tolerance in ovariectomized rats and to compare them to the effects of 17beta-estradiol.
Twenty-eight female Sprague-Dawley rats were ovariectomized and fed soy-free chow with the addition of estradiol-3 benzoate (E2B) (10 mg/kg, n=10) or equol (400 mg/kg, n=10). The control group (n=8) received soy-free chow only. Weight and food intake were recorded once weekly. After 6 weeks, intra-abdominal fat was measured using computed tomography, and the intraperitoneal glucose tolerance test was performed. In the seventh week, the animals were killed, blood was collected for plasma, and uteri were removed.
Dietary equol significantly increased uterine mass. This effect was, however, 3.5 times lower in magnitude compared to E2B. Similar to E2B, dietary equol decreased weight gain, intra-abdominal fat accumulation, and plasma leptin levels. Equol-treated animals had also lower plasma total cholesterol and triglyceride levels compared to controls. E2B treatment also decreased plasma total cholesterol as well as high-density lipoprotein and low-density lipoprotein cholesterol. In the glucose tolerance test, the area under the curve was significantly smaller in the E2B- and equol-treated animals compared to controls. Also, E2B-treated animals had lower fasting plasma insulin levels.
In ovariectomized rats, dietary equol administration attenuates weight gain and shows favorable metabolic effects. However, because of its mild uterotrophic activity, its use in the prevention of postmenopausal weight gain and related metabolic disorders in women with an intact uterus is questionable in terms of safety and warrants further studies.
评估植物雌激素黄豆苷元的代谢产物雌马酚对去卵巢大鼠体重增加、腹内脂肪堆积、血浆瘦素、脂质及葡萄糖耐量的影响，并将其与17β-雌二醇的作用进行比较。
将28只雌性Sprague-Dawley大鼠去卵巢，喂食不含大豆的食物，并添加苯甲酸雌二醇（E2B）（10毫克/千克，n = 10）或雌马酚（400毫克/千克，n = 10）。对照组（n = 8）仅喂食不含大豆的食物。每周记录一次体重和食物摄入量。6周后，使用计算机断层扫描测量腹内脂肪，并进行腹腔葡萄糖耐量试验。在第7周，处死动物，采集血液获取血浆，并摘除子宫。
饮食中的雌马酚显著增加子宫重量。然而，与E2B相比，这种作用的强度低3.5倍。与E2B相似，饮食中的雌马酚减少了体重增加、腹内脂肪堆积和血浆瘦素水平。与对照组相比，接受雌马酚治疗的动物血浆总胆固醇和甘油三酯水平也较低。E2B治疗还降低了血浆总胆固醇以及高密度脂蛋白和低密度脂蛋白胆固醇。在葡萄糖耐量试验中，与对照组相比，接受E2B和雌马酚治疗的动物曲线下面积显著更小。此外，接受E2B治疗的动物空腹血浆胰岛素水平较低。
在去卵巢大鼠中，饮食中给予雌马酚可减轻体重增加并显示出有利的代谢作用。然而，由于其轻微的子宫营养活性，就安全性而言，其用于预防子宫完整的女性绝经后体重增加及相关代谢紊乱存在疑问，值得进一步研究。
