Queiroga F L, Alves A, Pires I, Lopes C
Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-911, Vila Real, Portugal.
J Comp Pathol. 2007 Feb-Apr;136(2-3):177-85. doi: 10.1016/j.jcpa.2007.01.010.
The aim of this study was to investigate immunohistochemically the expression of cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) in canine mammary tumours of different histological types. Cox-1 and Cox-2 enzyme expression was evaluated in 70 mammary samples (four normal, six hyperplastic, 60 neoplastic [21 benign and 39 malignant]). Cox-1 expression was identified in all the samples, and Cox-2 in all the mammary lesions except ductal hyperplasia. Two of the four normal mammary gland samples showed focal immunoreactivity for Cox-2. Cox-1 immunoexpression did not differ significantly between benign and malignant lesions (P=0.272). Cox-2 immunoexpression was higher in malignant tumours than in benign counterparts (P<0.001). Of the malignant tumours, carcinosarcomas and tubulopapillary and squamous cell carcinomas had the highest Cox-2 scores. The study showed that malignant tumours had the highest values of Cox-2 expression, and Cox-2 immunolabelling was particularly intense in histological types classically associated with high malignancy. This suggests that nonsteroidal anti-inflammatory drugs (NSAIDs), particularly Cox-2 inhibitors, may have a useful role to play in the treatment of canine malignant mammary tumours.
本研究的目的是通过免疫组织化学方法研究不同组织学类型犬乳腺肿瘤中环氧化酶-1（Cox-1）和环氧化酶-2（Cox-2）的表达。在70个乳腺样本（4个正常样本、6个增生样本、60个肿瘤样本[21个良性和39个恶性]）中评估Cox-1和Cox-2酶的表达。在所有样本中均检测到Cox-1的表达，除导管增生外的所有乳腺病变中均检测到Cox-2的表达。4个正常乳腺样本中有2个显示出Cox-2的局灶性免疫反应性。良性和恶性病变之间的Cox-1免疫表达无显著差异（P=0.272）。恶性肿瘤中的Cox-2免疫表达高于良性肿瘤（P<0.001）。在恶性肿瘤中，癌肉瘤、管状乳头状癌和鳞状细胞癌的Cox-2评分最高。该研究表明，恶性肿瘤的Cox-2表达值最高，并且在经典的高恶性组织学类型中Cox-2免疫标记特别强烈。这表明非甾体抗炎药（NSAIDs），尤其是Cox-2抑制剂，可能在犬恶性乳腺肿瘤的治疗中发挥有益作用。
