Yagmurca Murat, Bas Orhan, Mollaoglu Hakan, Sahin Onder, Nacar Ahmet, Karaman Ozcan, Songur Ahmet
Histology-Embryology, Afyon Kocatepe University, Medical Faculty, Afyonkarahisar, Turkey.
Arch Med Res. 2007 May;38(4):380-5. doi: 10.1016/j.arcmed.2007.01.007.
Oxidative stress has an important role in the pathogenesis of doxorubicin-induced hepatotoxicity. The aim of this study was to investigate the hepatoprotective effects of erdosteine, an antioxidant agent, on doxorubicin-induced hepatotoxicity.
Rats were divided into control, doxorubicin alone (20 mg/kg, i.p.) and doxorubicin plus erdosteine (50 mg/kg/day, oral) groups. At the end of the 10(th) day, liver tissues were removed for light microscopy and analysis. The levels of tissue protein carbonyl content, malondialdehyde and nitric oxide, as well as the activities of superoxide dismutase, catalase, were determined.
The tissue of the doxorubicin group showed some histopathological changes such as necrosis, hepatocyte degeneration, sinusoidal dilatation, hemorrhage and vascular congestion and dilatation. In the doxorubicin plus erdosteine group, histopathological evidence of hepatic damage was markedly reduced. Biochemical parameters were consistent with histological parameters.
Doxorubicin caused hepatotoxicity, and erdosteine treatment prevented lipid peroxidation and protein oxidant in liver tissue.
氧化应激在阿霉素诱导的肝毒性发病机制中起重要作用。本研究旨在探讨抗氧化剂厄多司坦对阿霉素诱导的肝毒性的肝保护作用。
将大鼠分为对照组、单独给予阿霉素组（20mg/kg，腹腔注射）和阿霉素加厄多司坦组（50mg/kg/天，口服）。在第10天结束时，取出肝脏组织进行光学显微镜检查和分析。测定组织蛋白羰基含量、丙二醛和一氧化氮水平，以及超氧化物歧化酶、过氧化氢酶的活性。
阿霉素组组织出现一些组织病理学变化，如坏死、肝细胞变性、窦状隙扩张、出血以及血管充血和扩张。在阿霉素加厄多司坦组，肝损伤的组织病理学证据明显减轻。生化参数与组织学参数一致。
阿霉素引起肝毒性，而厄多司坦治疗可预防肝组织中的脂质过氧化和蛋白质氧化。
