Motamedi Rezvan, Aminzadeh Soheila, Khodayar Mohammad Javad, Khorsandi Layasadat, Salehcheh Maryam
Toxicol Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Toxicol, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Rep Biochem Mol Biol. 2024 Jan;12(4):575-585. doi: 10.61186/rbmb.12.4.575.
Doxorubicin, a commonly utilized anthracycline antibiotic and chemotherapeutic agent, has been associated with hepatotoxicity as an adverse effect. This study aimed to evaluate protective effects of zingerone, a bioactive compound derived from ginger renowned for its antioxidative attributes, on oxidative stress in doxorubicin-induced rat hepatotoxicity.
In this experimental study, a total of 48 male Wistar rats were allocated into six distinct groups. The first group received a control treatment of normal saline. The second group was administered an intraperitoneal dose of 20 mg/kg of doxorubicin on day 5. The third group received an oral dose of 40 mg/kg of zingerone for 8 days. The fourth, fifth, and sixth groups were administered zingerone at doses of 10, 20, and 40 mg/kg, respectively, for the same 8-day period. On day 5, all groups, except the control group, received an intraperitoneal injection of doxorubicin. Following a 72-hour interval, the animals were anesthetized, and blood samples were collected to assess serum factors. Moreover, portions of the liver tissue were subjected to histopathological analysis and assessment of oxidative stress parameters.
The activity levels of serum enzymes, including aspartate transaminase (AST), alanine transaminase (ALT), and liver malondialdehyde (MDA), increased in the doxorubicin group. Conversely, the levels of other parameters such as glutathione peroxidase (GPX), superoxide dismutase (SOD), and glutathione (GSH) decreased. However, the co-administration of zingerone effectively reversed these levels, restoring them back to normal.
These findings suggest that zingerone, particularly at a high dose, exhibit a hepatoprotective effect in the doxorubicin-induced hepatotoxicity model.
阿霉素是一种常用的蒽环类抗生素和化疗药物，其不良反应与肝毒性有关。本研究旨在评估姜辣素（一种源自生姜的具有抗氧化特性的生物活性化合物）对阿霉素诱导的大鼠肝毒性氧化应激的保护作用。
在本实验研究中，总共48只雄性Wistar大鼠被分为六个不同的组。第一组接受生理盐水对照处理。第二组在第5天腹腔注射20mg/kg的阿霉素。第三组口服40mg/kg的姜辣素，持续8天。第四、第五和第六组在相同的8天时间内分别给予10mg/kg、20mg/kg和40mg/kg的姜辣素。在第5天，除对照组外，所有组均腹腔注射阿霉素。72小时后，将动物麻醉，采集血样以评估血清因子。此外，取部分肝组织进行组织病理学分析和氧化应激参数评估。
阿霉素组血清酶（包括天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT））和肝丙二醛（MDA）的活性水平升高。相反，其他参数如谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平降低。然而，姜辣素的联合给药有效地逆转了这些水平，使其恢复正常。
这些发现表明，姜辣素，特别是高剂量时，在阿霉素诱导的肝毒性模型中具有肝保护作用。
