Nakahodo K, Nakamura M, Kosugi T, Ura M, Taira A
Department of Physiology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Auris Nasus Larynx. 1991;18(2):145-51. doi: 10.1016/s0385-8146(12)80218-5.
As an experimental model of human tonsillitis, Arthus tonsillitis of the rabbit was produced. In a previous paper, we demonstrated that an increased fibrinolytic activity in the circulating blood and increased proteolytic activity of the local tissue with Arthus tonsillitis were observed. In the present study, the mechanism of the increased proteolytic activity at the local site (tonsil with Arthus-type tonsillitis) was examined using cell cultures of lymphocytes originating from the tonsil with Arthus-type inflammation. That is, lymphocytes isolated from tonsil tissue of the rabbit were cultured in a culture medium for the short term. After culture, the used medium was collected and the presence of plasminogen activator in the culture medium and lymphocytes was investigated. It was clarified that plasminogen activator did exist in the culture medium of lymphocytes originating from the tonsil of rabbits with Arthus tonsillitis. Furthermore, addition of T cell growth factor to the culture medium of lymphocytes derived from non-treated tonsil induced the development of plasminogen activator in that culture medium. However, addition of B cell growth factor did not lead to the development of plasminogen activator activity in the culture medium. These results suggest that plasminogen activator originating from lymphocytes of the tonsil may play an important role in the increased proteolytic activity observed at the local site of Arthus tonsillitis.
作为人类扁桃体炎的实验模型，制作了兔的阿瑟斯扁桃体炎模型。在之前的一篇论文中，我们证明了在阿瑟斯扁桃体炎中观察到循环血液中纤维蛋白溶解活性增加以及局部组织的蛋白水解活性增加。在本研究中，使用源自患有阿瑟斯型炎症扁桃体的淋巴细胞进行细胞培养，研究了局部部位（阿瑟斯型扁桃体炎的扁桃体）蛋白水解活性增加的机制。也就是说，从兔扁桃体组织分离的淋巴细胞在培养基中进行短期培养。培养后，收集用过的培养基，研究培养基和淋巴细胞中纤溶酶原激活物的存在情况。结果表明，患有阿瑟斯扁桃体炎的兔扁桃体来源的淋巴细胞培养基中确实存在纤溶酶原激活物。此外，向未处理扁桃体来源的淋巴细胞培养基中添加T细胞生长因子可诱导该培养基中纤溶酶原激活物的产生。然而，添加B细胞生长因子并不会导致培养基中纤溶酶原激活物活性的产生。这些结果表明，源自扁桃体淋巴细胞的纤溶酶原激活物可能在阿瑟斯扁桃体炎局部部位观察到的蛋白水解活性增加中起重要作用。
