Chen Yanfei, Chen Jishuang, Zhang Huarong, Tang Xiangshan, Du Zhiyou
Institute of Bioengineering, Zhejiang Sci-Tech University, No.2 Road Xiasha, Hangzhou, Zhejiang 310018, P R China.
Virus Genes. 2007 Oct;35(2):405-13. doi: 10.1007/s11262-007-0094-z. Epub 2007 Apr 7.
Cucumber mosaic virus (CMV) is a tripartite RNA virus and has been divided into three subgroups, named IA, IB, and II. Some studies have found a few natural reassortants between CMV subgroups, although reassortment between CMV subgroups is infrequent. In our present work, a CMV reassortant, named CMV-Tsh, was obtained from a tomato plant. The complete sequence of CMV-Tsh genomic RNAs has been determined and analyzed. The results of sequence comparisons and phylogenetic analyses revealed that CMV-Tsh RNAs 1 and 3 are derived from one or two CMV subgroup II strain(s), while RNA2 is derived from a CMV subgroup IA strain. A PCR and restriction enzyme analysis-based method was developed to analyze the possibility of mixed infection by CMV strains of different subgroup in the CMV-Tsh-infected tomato plant. The results of the restriction enzyme analysis proved that CMV-Tsh is the unique strain in the tomato plant. Taken together, CMV-Tsh is a natural reassortant having CMV subgroup IA RNA2 and subgroup II RNAs 1 and 3.
黄瓜花叶病毒（CMV）是一种三分体RNA病毒，已被分为三个亚组，命名为IA、IB和II。一些研究发现CMV亚组之间存在少数天然重配体，尽管CMV亚组之间的重配很少见。在我们目前的工作中，从一株番茄植株中获得了一种CMV重配体，命名为CMV-Tsh。已确定并分析了CMV-Tsh基因组RNA的完整序列。序列比较和系统发育分析结果表明，CMV-Tsh的RNA1和RNA3来源于一个或两个CMV亚组II毒株，而RNA2来源于CMV亚组IA毒株。开发了一种基于PCR和限制酶分析的方法，以分析CMV-Tsh感染的番茄植株中不同亚组CMV毒株混合感染的可能性。限制酶分析结果证明CMV-Tsh是番茄植株中的独特毒株。综上所述，CMV-Tsh是一种具有CMV亚组IA RNA2和亚组II RNA1和RNA3的天然重配体。
