Phytopathology. 1998 Feb;88(2):92-7. doi: 10.1094/PHYTO.1998.88.2.92.
ABSTRACT In Northern hybridization assays, RNA1 of peanut stunt virus (PSV) strain BV-15 hybridized strongly with a cloned cDNA probe to RNA1 from strain PSV-W (subgroup II). Cloned probes to PSV-W RNA2 and RNA3, however, did not hybridize with the corresponding RNAs from strain BV-15. The complete nucleotide sequence of PSV-BV-15 RNA2 has been determined, and sequence comparison analysis showed that it is closely related to PSV subgroup I strains; the percent nucleotide sequence identity between PSV-BV-15 RNA2 and RNA2 sequences from PSV subgroup I and II strains were 90.5 and 75%, respectively. The possibility that PSV-BV-15 RNA2 may contain short regions derived from a subgroup II strain (i.e., represent a mosaic structure indicative of recombination) was investigated. Results indicated, however, that the entire PSV-BV-15 RNA2 sequence is derived from a subgroup I strain. PSV-BV-15 RNA3 has previously been shown to belong to subgroup I strains. These results thus establish that PSV strain BV-15 is a natural reassortant between PSV subgroups I and II strains. A reverse transcription-po-lymerase chain reaction assay is proposed for differentiating between this reassortant strain and PSV strains in subgroups I and II.
摘要 在北方杂交分析中，花生丛矮病毒（PSV）BV-15 株的 RNA1 与 RNA1 克隆 cDNA 探针强烈杂交来自 PSV-W 株（亚组 II）。然而，PSV-W RNA2 和 RNA3 的克隆探针并未与来自 BV-15 株的相应 RNA 杂交。PSV-BV-15 RNA2 的完整核苷酸序列已被确定，序列比较分析表明它与 PSV 亚组 I 株密切相关；PSV-BV-15 RNA2 与 PSV 亚组 I 和 II 株的 RNA2 序列的核苷酸序列同一性分别为 90.5%和 75%。PSV-BV-15 RNA2 可能含有来自亚组 II 株的短区域（即，代表重组的镶嵌结构）的可能性进行了调查。然而，结果表明，整个 PSV-BV-15 RNA2 序列均来自亚组 I 株。PSV-BV-15 RNA3 先前已被证明属于亚组 I 株。这些结果表明，PSV 株 BV-15 是 PSV 亚组 I 和 II 株之间的自然重组体。提出了一种逆转录聚合酶链反应（RT-PCR）检测方法，用于区分该重组体菌株和亚组 I 和 II 的 PSV 菌株。
