Pepe Tiziana, Trotta Michele, Di Marco Isolina, Anastasio Aniello, Bautista José Manuel, Cortesi Maria Luisa
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Falcoltà di Medicina Veterinaria, Università Federico II, Napoli, Italy.
J Agric Food Chem. 2007 May 2;55(9):3681-5. doi: 10.1021/jf063321o. Epub 2007 Apr 7.
Whole fish morphologically identified as belonging to Theragra chalcogramma, Merluccius merluccius, Merluccius hubbsi, and Merluccius capensis and 19 fish products commercialized as surimi with different commercial brands and labeled as T. chalcogramma were analyzed by direct sequence analysis of the cytochrome b gene. A phylogenetic analysis of surimi products was performed as well. Results demonstrated that mislabeling is a large-scale phenomenon, since 84.2% of surimi-based fish products sold as T. chalcogramma (16/19) were prepared with species different from the one declared. In fact, only three samples (samples 15-17) were found to belong to T. chalcogramma. In the remaining samples, Merluccidae (samples 4-14), Gadidae (samples 18 and 19), Sparidae (sample 1), and Pomacentridae (samples 2 and 3) families were detected. A phylogenetic tree was constructed, and the bootstrap value was calculated. According to this methodology, 11 samples were grouped in the same clade as Merluccius spp.
对形态学上鉴定为属于黄线狭鳕、无须鳕、哈氏无须鳕和南非无须鳕的整条鱼，以及19种以不同商业品牌商业化销售并标记为黄线狭鳕的鱼糜制品进行了细胞色素b基因的直接序列分析。同时对鱼糜制品进行了系统发育分析。结果表明，标签错误是一个大规模现象，因为以黄线狭鳕销售的鱼糜基鱼制品中有84.2%（16/19）是用与宣称的物种不同的物种制备的。事实上，仅发现三个样本（样本15 - 17）属于黄线狭鳕。在其余样本中，检测到了无须鳕科（样本4 - 14）、鳕科（样本18和19）、鲷科（样本1）和雀鲷科（样本2和3）。构建了系统发育树并计算了自展值。根据该方法，11个样本与无须鳕属归为同一分支。
