Mascolo Celestina, Ceruso Marina, Sordino Paolo, Palma Giuseppe, Anastasio Aniello, Pepe Tiziana
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Biology and Evolution of Marine Organisms, Research Centre Anton Dohrn Zoological Station, Naples, Italy.
Ital J Food Saf. 2017 Jun 22;6(2):6154. doi: 10.4081/ijfs.2017.6154. eCollection 2017 Apr 13.
Previous studies showed that fish mitochondrial DNA (mtDNA) is set up by a closed circular molecule of 16-17 kilobases (kb), comprising 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), 13 protein-coding genes and 2 non-coding regions. The analysis of single mtDNA genes, such as Cytb, COI, 16S and 12S, or short segment of them, has been widely used against species substitution in both fresh and processed fish products. The analysis of the complete mitochondrial genome of fishery products allows to better study and characterise fish species. The aim of this research was to extract and amplify the complete mtDNA of some fish species of commercial interest belonging to the Sparidae family. The studied species were and The entire mitogenome was obtained by gene amplification using long polymerase chain reactions. The analysis of the complete mitochondrial sequences will allow to gain further insights on these species and to find polymorphic sites that assess the degree of genetic variability of the species belonging to the family Sparidae.
先前的研究表明，鱼类线粒体DNA（mtDNA）由一个16 - 17千碱基（kb）的闭环分子组成，包含2个核糖体RNA基因（rRNA）、22个转移RNA基因（tRNA）、13个蛋白质编码基因和2个非编码区。对单个mtDNA基因（如Cytb、COI、16S和12S）或其短片段的分析已广泛用于检测新鲜和加工鱼类产品中的物种替代情况。对渔业产品完整线粒体基因组的分析有助于更好地研究和鉴定鱼类物种。本研究的目的是提取和扩增一些属于鲷科的具有商业价值的鱼类物种的完整mtDNA。所研究的物种为[具体物种1]和[具体物种2]。通过使用长聚合酶链反应进行基因扩增获得了完整的有丝分裂基因组。对完整线粒体序列的分析将有助于深入了解这些物种，并找到多态性位点，以评估鲷科物种的遗传变异程度。
