系统性硬化症中循环内皮祖细胞的水平

Levels of circulating endothelial progenitor cells in systemic sclerosis.

作者信息

Allanore Y, Batteux F, Avouac J, Assous N, Weill B, Kahan A

机构信息

Rheumatology A and Immunology departments, René Descartes University, Medical School, Cochin Hospital, Assistance Publique Hôpitaux de Paris, France.

出版信息

Clin Exp Rheumatol. 2007 Jan-Feb;25(1):60-6.

PMID:17417992

Abstract

OBJECTIVE

Contradictory results have been reported regarding vasculogenesis in systemic sclerosis (SSc). Our aim was to investigate bone marrow-derived circulating endothelial precursors (EPCs) and activated circulating endothelial cells (CECs) in SSc patients.

METHODS

Peripheral blood from consecutive patients with SSc hospitalised for systemic follow-up was analysed and compared with blood from patients with active refractory rheumatoid arthritis (RA) and osteoarthritis (OA). EPCs were quantified by cell sorting and flow cytometry and were identified as circulating CD34+CD133+ cells. Activated CECs were defined as CD105+CD62+CD105+CD102+CD105+CD106+ cells.

RESULTS

Patients with SSc had higher putative EPC levels than OA patients, but lower levels than RA patients. In SSc patients, EPC levels increased with European disease activity score. Activated CEC levels were high in SSc patients and RA patients, but not correlated with EPC levels.

CONCLUSION

These results together and previous data suggest that EPCs may be recruited during active vascular disease but that the sustained ischaemic conditions of SSc may eventually lead to EPCs depletion.

摘要

目的

关于系统性硬化症（SSc）中的血管生成，已有相互矛盾的研究结果报道。我们的目的是研究SSc患者骨髓来源的循环内皮祖细胞（EPCs）和活化的循环内皮细胞（CECs）。

方法

分析因全身随访而住院的连续性SSc患者的外周血，并与活动性难治性类风湿关节炎（RA）和骨关节炎（OA）患者的血液进行比较。通过细胞分选和流式细胞术对EPCs进行定量，并将其鉴定为循环CD34+CD133+细胞。活化的CECs定义为CD105+CD62+CD105+CD102+CD105+CD106+细胞。