Allanore Y, Batteux F, Avouac J, Assous N, Weill B, Kahan A
Rheumatology A and Immunology departments, René Descartes University, Medical School, Cochin Hospital, Assistance Publique Hôpitaux de Paris, France.
Clin Exp Rheumatol. 2007 Jan-Feb;25(1):60-6.
Contradictory results have been reported regarding vasculogenesis in systemic sclerosis (SSc). Our aim was to investigate bone marrow-derived circulating endothelial precursors (EPCs) and activated circulating endothelial cells (CECs) in SSc patients.
Peripheral blood from consecutive patients with SSc hospitalised for systemic follow-up was analysed and compared with blood from patients with active refractory rheumatoid arthritis (RA) and osteoarthritis (OA). EPCs were quantified by cell sorting and flow cytometry and were identified as circulating CD34+CD133+ cells. Activated CECs were defined as CD105+CD62+CD105+CD102+CD105+CD106+ cells.
Patients with SSc had higher putative EPC levels than OA patients, but lower levels than RA patients. In SSc patients, EPC levels increased with European disease activity score. Activated CEC levels were high in SSc patients and RA patients, but not correlated with EPC levels.
These results together and previous data suggest that EPCs may be recruited during active vascular disease but that the sustained ischaemic conditions of SSc may eventually lead to EPCs depletion.
关于系统性硬化症（SSc）中的血管生成，已有相互矛盾的研究结果报道。我们的目的是研究SSc患者骨髓来源的循环内皮祖细胞（EPCs）和活化的循环内皮细胞（CECs）。
分析因全身随访而住院的连续性SSc患者的外周血，并与活动性难治性类风湿关节炎（RA）和骨关节炎（OA）患者的血液进行比较。通过细胞分选和流式细胞术对EPCs进行定量，并将其鉴定为循环CD34+CD133+细胞。活化的CECs定义为CD105+CD62+CD105+CD102+CD105+CD106+细胞。
SSc患者的假定EPC水平高于OA患者，但低于RA患者。在SSc患者中，EPC水平随欧洲疾病活动评分增加。SSc患者和RA患者的活化CEC水平较高，但与EPC水平无关。
这些结果以及先前的数据表明，EPCs可能在活动性血管疾病期间被募集，但SSc的持续缺血状态最终可能导致EPCs耗竭。
