[中老年高脂血症患者血小板磷脂脂肪酸与血栓形成危险因素的关系]

[Relations of platelet phospholipid fatty acids to thrombotic risk factors in middle-aged and geriatric patients with hyperlipidemia].

作者信息

Yu Xiao-Mei, Li Duo, Xie Hai-Bao, Zhang Yong-Hua, Wang Qiang, Zhou Xiao-Qiong, Yu Peng, Wang Li-Jun

机构信息

Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 Jan 9;87(2):105-8.

PMID:17418017

Abstract

OBJECTIVE

To investigate the relations of platelet phospholipid fatty acids to thrombotic risk factors in the middle-aged and geriatric patients with hyperlipidemia in the metropolitan area of Hangzhou, Zhejiang province.

METHODS

A questionnaire survey was conducted among 81 patients with hyperlipidemia, 50 males and 31 females, aged (57 +/- 8), and 65 healthy controls, 43 males and 22 females, aged (58 +/- 9) to collect the data about height, weight, and diet. Peripheral venous blood samples were collected to examine the total cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha)), and thromboxane B(2) (TXB(2)) were examined by standard methods. Serum thrombotic risk factors including homocysteine and Thromboxane B(2) were determined by standard methods. Platelet phospholipid fatty acids were examined by gas chromatography. The correlation between the serum thrombotic risk factors (Hcy, TXB(2), and 6-keto-PG F1a) was analyzed by multivariate linear regression.

RESULTS

There were no significant differences in platelet phospholipid fatty acids between the patients with hyperlipidemia and the healthy controls. Serum Hcy was significantly negatively correlated with docosahexaenoic acid (DHA) and the ratio of n-3 PUFA (polyunsaturated fatty acids)/n-6 PUFA (r = -0.277 and -0.231, both P < 0.01). The level of serum TXB(2) was significantly positively correlated with arachidonic acid (r = 0.176, P < 0.05), and significantly negatively correlated with DHA (r = -0.209, P < 0.01), eicosapentaenoic acid (EPA) (r = -0.194, P < 0.05), and n-3 PUFA/n-6 PUFA (r = -0.238, P < 0.01).

CONCLUSION

Increasing the ratio of n-3 PUFA/n-6 PUFA in platelet phospholipid may potentially decrease the thrombotic risks such as Hcy and TXB(2) and provide a reference for diet selection.

摘要

目的

探讨浙江省杭州市城区中老年高脂血症患者血小板磷脂脂肪酸与血栓形成危险因素的关系。

方法

对81例高脂血症患者（年龄57±8岁，男性50例，女性31例）和65例健康对照者（年龄58±9岁，男性43例，女性22例）进行问卷调查，收集身高、体重和饮食等数据。采集外周静脉血样本，采用标准方法检测总胆固醇、甘油三酯、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、同型半胱氨酸（Hcy）、6-酮-前列腺素F（1α）（6-keto-PGF（1α））和血栓素B（2）（TXB（2））。采用标准方法测定血清血栓形成危险因素，包括同型半胱氨酸和血栓素B（2）。采用气相色谱法检测血小板磷脂脂肪酸。通过多元线性回归分析血清血栓形成危险因素（Hcy、TXB（2）和6-酮-PG F1a）之间的相关性。

结果

高脂血症患者与健康对照者的血小板磷脂脂肪酸无显著差异。血清Hcy与二十二碳六烯酸（DHA）及n-3多不饱和脂肪酸（PUFA）/n-6多不饱和脂肪酸比值呈显著负相关（r = -0.277和-0.231，P均<0.01）。血清TXB（2）水平与花生四烯酸呈显著正相关（r = 0.176，P < 0.05），与DHA（r = -0.209，P < 0.01）、二十碳五烯酸（EPA）（r = -0.194, P < 0.05）及n-3 PUFA/n-6 PUFA呈显著负相关（r = -0.238, P < 0.01）。