Hostanska Katarina, Jürgenliemk Guido, Abel Gudrun, Nahrstedt Adolf, Saller Reinhard
University Hospital Zürich, Department of Internal Medicine, Institute for Complementary Medicine, FGel 102, Rämistrasse 100, CH-8091 Zürich, Switzerland.
Cancer Detect Prev. 2007;31(2):129-39. doi: 10.1016/j.cdp.2007.03.001. Epub 2007 Apr 6.
Recently, there have been extensive efforts to evaluate the chemopreventive role of substances present in natural products. The aim of this study was to examine the effects of the main groups of compounds (salicylalcohol derivates, flavonoids, proanthocyanidins), and salicin isolated from willow bark extract BNO 1455 on proliferation and apoptosis in human colon and cancer cells.
We used human colon cyclooxygenase-2 (COX-2)-positive HT 29 and (COX-2)-negative HCT 116 or lung COX-2 proficient A 549 and low COX-2 expressing SW2 cells. After treatment for 72 h with various concentrations of single substances and acetylsalicylic acid (ASA) as control, inhibition of cell growth and cytotoxicity were measured by colorimetric WST-1 assay and propidium iodide uptake by flow cytometry, respectively. Apoptotic cells were identified by annexin V adhesion using flow cytometry.
Studies on dose-dependent effects of BNO 1455 and its fractions showed anti-proliferative activity of all compounds with 50% maximal growth inhibitory concentrations (GI(50)) between 33.3 and 103.3 microg/ml for flavonoids and proanthocyanidins fractions and 50.0-243.0 microg/ml for salicylalcohol derivates and extract. Apoptosis induction was confirmed by annexin V adherence and analysis of cell morphology based on light scattering characteristics using flow cytometry in all cell lines at GI(50).
We showed that willow bark extract BNO 1455 an its fractions inhibit the cell growth and promote apoptosis in human colon and lung cancer cell lines irrespective of their COX-selectivity.
最近，人们为评估天然产物中物质的化学预防作用付出了巨大努力。本研究的目的是考察从柳树皮提取物BNO 1455中分离出的主要化合物组（水杨醇衍生物、黄酮类化合物、原花青素）以及水杨苷对人结肠癌细胞和肺癌细胞增殖及凋亡的影响。
我们使用人结肠环氧合酶-2（COX-2）阳性的HT 29细胞和（COX-2）阴性的HCT 116细胞，或肺COX-2高表达的A 549细胞和低COX-2表达的SW2细胞。用不同浓度的单一物质和作为对照的乙酰水杨酸（ASA）处理72小时后，分别通过比色法WST-1测定法和流式细胞术检测碘化丙啶摄取来测量细胞生长抑制和细胞毒性。通过流式细胞术使用膜联蛋白V黏附鉴定凋亡细胞。
对BNO 1455及其组分的剂量依赖性效应研究表明，所有化合物均具有抗增殖活性，黄酮类化合物和原花青素组分的50%最大生长抑制浓度（GI(50)）在33.3至103.3微克/毫升之间，水杨醇衍生物和提取物的GI(50)在50.0至243.0微克/毫升之间。在GI(50)时，通过膜联蛋白V黏附以及基于流式细胞术光散射特性的细胞形态分析，在所有细胞系中均证实了凋亡诱导。
我们表明，柳树皮提取物BNO 1455及其组分可抑制人结肠癌细胞和肺癌细胞系的细胞生长并促进凋亡，而不论其COX选择性如何。
