Yang Shengjie, Liu Mingchuan, Zhao Qi, Zhao Hongju, Xue Wei, Yang Song
1State key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025 People's Republic of China.
Research Institute, Sinphar Tian-Li Pharmaceutical Co., Ltd, Hangzhou, 311100 People's Republic of China.
Med Chem Res. 2015;24(4):1644-1652. doi: 10.1007/s00044-014-1244-1. Epub 2014 Sep 4.
(J.) Smith is an endemic species in China, which has been proved to be an important Chinese herbal medicine. However, chemical composition and bioactivity of essential oil (EO) of (J.) Smith leaves remain unclear. In present study, we isolated EO from the plant by supercritical carbon dioxide extraction assay (SFE-CO), and investigated on cancer cells MGC-803, MCF-7, BGC-823, Bcap-37, A375, and A549 in vitro by MTT assay. 26 compounds were identified by GC-MS analysis, and the EO showed significant antitumor activities against MGC-803, Bcap-37, and A549 cancer cell lines (IC values ranging from 0.15 to 0.24 mg/mL), and the activities of its main component were also studied. Subsequent fluorescence staining and flow cytometry analysis indicated that the EO could induce apoptosis in MGC-803, Bcap-37, and A549 cell lines, and the apoptosis ratios reached 26.44 % after 48 h of treatment at 0.15 mg/mL in MGC-803 cells. Caspase 3 activity in MGC-803 cells was also determined when the cells treated with the oil, and the activity of caspase 3 enzyme was increased compared to the control. This study suggests that the EO isolated from (J.) Smith could inhibit the growth of human carcinoma cells, and it could induce apoptosis of cancer cells.
（J.）Smith是中国的特有物种，已被证明是一种重要的中药材。然而，（J.）Smith叶片精油（EO）的化学成分和生物活性仍不清楚。在本研究中，我们通过超临界二氧化碳萃取法（SFE-CO）从该植物中分离出EO，并通过MTT法在体外对癌细胞MGC-803、MCF-7、BGC-823、Bcap-37、A375和A549进行了研究。通过GC-MS分析鉴定出26种化合物，该EO对MGC-803、Bcap-37和A549癌细胞系表现出显著的抗肿瘤活性（IC值范围为0.15至0.24mg/mL），并对其主要成分的活性进行了研究。随后的荧光染色和流式细胞术分析表明，该EO可诱导MGC-803、Bcap-37和A549细胞系凋亡，在MGC-803细胞中以0.15mg/mL处理48小时后凋亡率达到26.44%。在用该油处理细胞时还测定了MGC-803细胞中半胱天冬酶3的活性，与对照相比，半胱天冬酶3的活性增加。本研究表明，从（J.）Smith中分离出的EO可抑制人癌细胞的生长，并可诱导癌细胞凋亡。
