Serefko Anna, Chudzik Beata, Malm Anna
Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodiki 1, 20-093 Lublin, Poland.
Pol J Microbiol. 2006;55(2):133-7.
Candida sp. may be regarded as one of the leading etiologic agents of hospital-acquired infections, including those related with the indwelling medical devices, which become colonized by the yeasts, accompanied by biofilm formation. In this paper we assayed in vitro susceptibility to caspofungin of planktonic and sessile cells of nasopharyngeal isolates of Candida sp. Two types of biomaterials were used - silicone elastomer-coated latex urinary Foley catheter and PCV Thorax catheter. The minimal inhibitory concentrations (MIC) of caspofungin for planktonic Candida sp. cells ranged from 0.008 to 0.031 mg/l, while the minimal fungicidal concentrations (MFC) from 0.008 to 0.062 mg/l, with MFC/MIC ratios < or = 2. The minimal concentration of caspofungin preventing adhesion process of Candida sp. on both biomaterials ranged from 0.004 to 0.031 mg/l, while preventing biofilm formation from 0.004 to 0.062 mg/l. In contrast, much higher minimal concentrations of caspofungin were needed to eradicate the mature biofilm (0.25 to >8 mg/l). In all cases, drug concentrations depended on the strain and the biomaterial used. Our preliminary data suggest that caspofungin, showing good anti-adherent activity in vitro against Candida sp., appears to be a potential agent rather for prophylaxis of the yeast infections associated with biomaterials but not for their treatment.
念珠菌属可能被视为医院获得性感染的主要病原体之一，包括与留置医疗设备相关的感染，这些设备会被酵母定植，并伴有生物膜形成。在本文中，我们检测了念珠菌属鼻咽分离株的浮游细胞和固着细胞对卡泊芬净的体外敏感性。使用了两种生物材料——硅胶弹性体涂层乳胶导尿管和聚氯乙烯胸段导管。卡泊芬净对浮游念珠菌属细胞的最低抑菌浓度（MIC）范围为0.008至0.031mg/l，而最低杀菌浓度（MFC）为0.008至0.062mg/l，MFC/MIC比值≤2。卡泊芬净防止念珠菌属在两种生物材料上黏附的最低浓度范围为0.004至0.031mg/l，而防止生物膜形成的最低浓度为0.004至0.062mg/l。相比之下，根除成熟生物膜需要更高的卡泊芬净最低浓度（0.25至>8mg/l）。在所有情况下，药物浓度取决于菌株和所用的生物材料。我们的初步数据表明，卡泊芬净在体外对念珠菌属显示出良好的抗黏附活性，似乎是预防与生物材料相关的酵母感染的潜在药物，但不是治疗此类感染的药物。
