Aoyagi Azusa, Yano Tatsuya, Kozuma Shiho, Takatsu Toshio
Core Technology Research Laboratories, Sankyo Co., Ltd., Hiromachi, Tokyo, Japan.
J Antibiot (Tokyo). 2007 Feb;60(2):143-52. doi: 10.1038/ja.2007.14.
Pleofungins (formerly called F-15078) A, B, C and D, novel depsipeptide antifungal antibiotics, were found in a mycelium extract of the producing fungus, Phoma sp. SANK 13899. The structures of pleofungins A, B, C and D were elucidated mainly by various NMR studies. The absolute configurations of the amino acids and N-methyl amino acids of pleofungin A constituents in the hydrolysate were determined by the application of advanced Marfey's method in combination with gas chromatography/mass spectrometry analysis of their silylation products with N-methyl-N-(tert-butylsilyl)trifluoroacetamide. Two alpha-hydroxy acid constituents, alpha-hydroxyisocaproic acid and alpha-hydroxyisovaleric acid, were isolated from the hydrolysate and their stereochemistries were determined by their specific rotations.
从产生真菌茎点霉属SANK 13899的菌丝体提取物中发现了新型缩肽类抗真菌抗生素多球霉素A、B、C和D（以前称为F - 15078）。多球霉素A、B、C和D的结构主要通过各种核磁共振研究得以阐明。通过先进的马尔费方法结合其与N - 甲基 - N -（叔丁基硅烷基）三氟乙酰胺的硅烷化产物的气相色谱/质谱分析，确定了水解产物中多球霉素A成分的氨基酸和N - 甲基氨基酸的绝对构型。从水解产物中分离出两种α - 羟基酸成分，α - 羟基异己酸和α - 羟基异戊酸，并通过它们的比旋光度确定了它们的立体化学结构。
