Ueno Akifumi, Hisatomi Toshio, Enaida Hiroshi, Kagimoto Tadahisa, Mochizuki Yasutaka, Goto Yoshinobu, Kubota Toshiaki, Hata Yasuaki, Ishibashi Tatsuro
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Retina. 2007 Apr-May;27(4):499-504. doi: 10.1097/IAE.0b013e318030a129.
To evaluate the toxicity of brilliant blue G (BBG) compared with those of indocyanine green (ICG) and trypan blue (TB) in a rat model of subretinal injection.
Retinal detachment was produced by subretinal injection of the dyes. The biocompatibility of BBG (0.25 mg/mL) was evaluated over 2 months and 2 weeks by ophthalmic examinations. The eyes were enucleated and analyzed by light, fluorescence, as well as transmission electron microscopy. Apoptotic cell death was detected by TdT-dUTP terminal nick-end labeling. The results were compared with those for ICG (5 mg/mL) and TB (1 mg/mL).
ICG caused retinal degeneration and retinal pigment epithelial (RPE) cell atrophy 2 weeks after subretinal injection. Apoptotic cell death was detected in the inner and outer nuclear layers and the RPE layer, especially the photoreceptors. TB caused less retinal degeneration, mainly in the area detached by the subretinal injection. BBG had no detectable toxic effects after 2 months and 2 weeks. Apoptotic cell death was detected in the ICG and TB groups, mainly in the photoreceptors.
Subretinal injection of the dyes caused retinal cell degeneration at lower concentrations than those reported for intravitreous injection. However, subretinal injection of BBG at 0.25 mg/mL appeared to provide satisfactory biocompatibility.
在大鼠视网膜下注射模型中，评估亮蓝G（BBG）与吲哚菁绿（ICG）和台盼蓝（TB）相比的毒性。
通过视网膜下注射染料造成视网膜脱离。通过眼科检查在2个月和2周的时间内评估BBG（0.25mg/mL）的生物相容性。摘除眼球并通过光镜、荧光显微镜以及透射电子显微镜进行分析。通过TdT-dUTP末端脱氧核苷酸转移酶介导的缺口末端标记法检测凋亡细胞死亡。将结果与ICG（5mg/mL）和TB（1mg/mL）的结果进行比较。
视网膜下注射2周后，ICG导致视网膜变性和视网膜色素上皮（RPE）细胞萎缩。在内核层、外核层以及RPE层，尤其是光感受器中检测到凋亡细胞死亡。TB导致的视网膜变性较轻，主要发生在视网膜下注射造成脱离的区域。2个月和2周后，BBG未检测到毒性作用。在ICG和TB组中检测到凋亡细胞死亡，主要发生在光感受器中。
视网膜下注射染料导致视网膜细胞变性的浓度低于玻璃体内注射报道的浓度。然而，视网膜下注射0.25mg/mL的BBG似乎具有令人满意的生物相容性。
