Kawaji Takahiro, Hirata Akira, Inomata Yasuya, Koga Takahisa, Tanihara Hidenobu
Department of Ophthalmology and Visual Science, Kumamoto University Graduate School of Medical Sciences, Honjo 1-1-1, 860-8556, Kumamoto , Japan.
Graefes Arch Clin Exp Ophthalmol. 2004 Feb;242(2):158-164. doi: 10.1007/s00417-003-0798-2. Epub 2004 Jan 14.
To investigate the effects of subretinal indocyanine green (ICG) on retinal morphology in rabbit eyes.
Retinal bleb detachments were produced by injections of ICG at dosages of 25 mg/ml, 5 mg/ml, and 0.5 mg/ml or with an injection of balanced salt solution (BSS) into the subretinal space of albino rabbit eyes. Morphological change was assessed by light and transmission electron microscopy from the viewpoint of dose and time. Some sections were also probed with the TUNEL technique to detect apoptotic cells.
At 14 days after subretinal injection of BSS and 0.5 mg/ml ICG, the structure of the retina was well preserved. However, injections of 5 mg/ml or 25 mg/ml caused thinning of the retina, especially loss of the outer retinal layer. In eyes injected with 5 mg/ml ICG, the photoreceptors began disappearing within 3 days after the injection and over time showed the development of retinal atrophy. TUNEL-positive cells appeared abundantly in the photoreceptor layers 1 and 3 days after the injection of 5 mg/ml ICG. Transmission electron microscopy confirmed apoptosis in the photoreceptors.
These data indicate that subretinal ICG induces apparent morphological damage of the retina in a dose-dependent manner.
研究视网膜下注射吲哚菁绿（ICG）对兔眼视网膜形态的影响。
通过向白化兔眼视网膜下间隙注射25mg/ml、5mg/ml和0.5mg/ml的ICG或平衡盐溶液（BSS），造成视网膜下液泡脱离。从剂量和时间的角度，通过光学显微镜和透射电子显微镜评估形态学变化。部分切片还用TUNEL技术检测凋亡细胞。
视网膜下注射BSS和0.5mg/ml ICG后14天，视网膜结构保存良好。然而，注射5mg/ml或25mg/ml会导致视网膜变薄，尤其是外层视网膜层的缺失。在注射5mg/ml ICG的眼中，光感受器在注射后3天内开始消失，随着时间的推移出现视网膜萎缩。注射5mg/ml ICG后1天和3天，光感受器层中大量出现TUNEL阳性细胞。透射电子显微镜证实光感受器存在凋亡。
这些数据表明，视网膜下注射ICG以剂量依赖性方式诱导视网膜明显的形态学损伤。
