Maia Mauricio, Kellner Lukas, de Juan Eugene, Smith Ricardo, Farah Michel E, Margalit Eyal, Lakhanpal Rohit R, Grebe Lynnea, Au Eong Kah-Guan, Humayun Mark S
Wilmer Ophthalmological Institute, The Johns Hopkins University School of Medicine, 550 North Broadway, Baltimore, MD 21205, USA.
Retina. 2004 Feb;24(1):80-91. doi: 10.1097/00006982-200402000-00012.
To evaluate the effects of indocyanine green (ICG) injection on the retinal surface and into the subretinal space of rabbit eyes.
Twenty-two Dutch-belted rabbits underwent two-port vitrectomy followed by injection of ICG (5 mg/mL) on the retinal surface and into the subretinal space. Balanced salt solution (BSS) was also injected subretinally. The locations where ICG was delivered (both epiretinal and subretinal) were exposed to light from an endoilluminator for 7 minutes. The animals were examined at 1, 7, and 14 days after surgery. The eyes were studied by fluorescein angiography as well as light and electron microscopy.
No damage was observed after epiretinal ICG injection, but subretinal ICG injection resulted in damage to the outer nuclear layer, photoreceptor inner and outer segments, and retinal pigment epithelium. This damage was more severe with longer follow-up. Control experiments without ICG, in which balanced salt solution was injected into the subretinal space or light was delivered on the epiretinal surface, demonstrated only damage to the photoreceptor outer segments.
Subretinal delivery of ICG (5 mg/mL) in rabbits induces retinal pigment epithelium, photoreceptor inner and outer segment, and outer nuclear layer damage. These mechanisms of damage may explain the retinal pigment epithelium changes that are sometimes seen after ICG-assisted internal limiting membrane peeling in humans.
评估吲哚菁绿（ICG）注射到兔眼视网膜表面和视网膜下间隙的效果。
22只荷兰带兔接受了双端口玻璃体切除术，随后将ICG（5mg/mL）注射到视网膜表面和视网膜下间隙。也将平衡盐溶液（BSS）注射到视网膜下。将ICG注射的部位（视网膜前和视网膜下）暴露于内照明器的光线下7分钟。在手术后1天、7天和14天对动物进行检查。通过荧光素血管造影以及光镜和电镜对眼睛进行研究。
视网膜前注射ICG后未观察到损伤，但视网膜下注射ICG导致外核层、光感受器内段和外段以及视网膜色素上皮受损。随着随访时间延长，这种损伤更严重。在不使用ICG的对照实验中，将平衡盐溶液注射到视网膜下间隙或在视网膜前表面照射光，仅显示光感受器外段受损。
在兔眼中视网膜下注射ICG（5mg/mL）会引起视网膜色素上皮、光感受器内段和外段以及外核层损伤。这些损伤机制可能解释了在人类ICG辅助内界膜剥除术后有时可见的视网膜色素上皮变化。
