Jonsson R, Pitts A, Mestecky J, Koopman W
Department of Oral Diagnosis, University of Göteborg, Sweden.
Autoimmunity. 1991;10(1):7-14. doi: 10.3109/08916939108997142.
Spontaneous local immunoglobulin (IgA, IgG, IgM) as well as IgA and IgM rheumatoid factor (RF) production in salivary glands, lymph nodes, and spleen was analyzed at various ages in autoimmune MRL/Mp-lpr/lpr (MRL/lpr) mice by using an ELISPOT assay. The longitudinal design of the study permitted correlations with severity of disease in salivary glands (sialadenitis). Local production of immunoglobulins in salivary glands and lymph nodes occurred with a pattern of IgG much greater than IgM greater than IgA. This isotype pattern differed from that simultaneously observed in spleen where IgG did not predominate to the same extent. Moreover, the spleen was the major site of IgM production. Rheumatoid factors constituted a significant fraction of local IgA and IgM in involved salivary glands. The pattern of IgA RF isotype expression in salivary glands contrasted with that observed in spleen. While the number of IgA and IgG secreting cells increase at an early age, the peak of RF production in salivary glands occurs in older mice. Furthermore, the level of immunoglobulin secretion was positively correlated with disease severity in salivary glands. The results suggest that local RF production is a secondary event in salivary gland inflammation in MRL/1pr mice rather than an initiating factor in this process.
通过使用酶联免疫斑点分析（ELISPOT），对自身免疫性MRL/Mp-lpr/lpr（MRL/lpr）小鼠不同年龄段的唾液腺、淋巴结和脾脏中自发产生的局部免疫球蛋白（IgA、IgG、IgM）以及IgA和IgM类风湿因子（RF）进行了分析。该研究的纵向设计允许将其与唾液腺（涎腺炎）疾病的严重程度相关联。唾液腺和淋巴结中免疫球蛋白的局部产生呈现出IgG远大于IgM大于IgA的模式。这种同种型模式与同时在脾脏中观察到的情况不同，在脾脏中IgG并不占主导地位。此外，脾脏是IgM产生的主要部位。类风湿因子在受累唾液腺的局部IgA和IgM中占很大比例。唾液腺中IgA RF同种型表达模式与在脾脏中观察到的不同。虽然分泌IgA和IgG的细胞数量在幼年时增加，但唾液腺中RF产生的峰值出现在老年小鼠中。此外，免疫球蛋白分泌水平与唾液腺疾病严重程度呈正相关。结果表明，局部RF产生是MRL/1pr小鼠唾液腺炎症中的继发事件，而非该过程的起始因素。
