Tarkowski A, Czerkinsky C, Nilsson L A
Clin Exp Immunol. 1984 Oct;58(1):7-12.
The kinetics of appearance of cells secreting IgG rheumatoid factor (IgG RF) has been studied in MRL/l mice by means of the ELISPOT assay, a new immunoenzyme procedure. Mice of the MRL/l strain spontaneously develop an autoimmune disease associated with arthritic manifestations. IgG RF secreting splenocytes were first detected at the clinical onset of the disease at the age of 3 months. Peak frequencies of IgG RF secreting cells amounting to almost 10% of the total number of IgG secreting cells were observed at later stages. The appearance of IgG RF secreting cells was preceded by at least 1 month by elevated levels of circulating immune complexes and abnormally high numbers of IgG secreting cells. The frequencies of IgG RF secreting cells and of IgG secreting splenocytes did not follow each other at least not during the first 3 months preceding the onset of clinical disease. The data presented suggest that IgG RF production in MRL/l mice is not triggered as part of an early stage of polyclonal activation but could be induced by IgG containing immune complexes.
通过一种新的免疫酶方法——酶联免疫斑点分析（ELISPOT 分析），对 MRL/l 小鼠分泌 IgG 类风湿因子（IgG RF）的细胞出现动力学进行了研究。MRL/l 品系小鼠会自发发展出一种与关节炎表现相关的自身免疫性疾病。分泌 IgG RF 的脾细胞在 3 个月龄疾病临床发作时首次被检测到。在疾病后期观察到分泌 IgG RF 的细胞的峰值频率几乎占分泌 IgG 细胞总数的 10%。在分泌 IgG RF 的细胞出现之前至少 1 个月，循环免疫复合物水平升高且分泌 IgG 的细胞数量异常增多。分泌 IgG RF 的细胞频率与分泌 IgG 的脾细胞频率并不相互跟随，至少在临床疾病发作前的前 3 个月不是这样。所呈现的数据表明，MRL/l 小鼠中 IgG RF 的产生不是作为多克隆激活早期阶段的一部分被触发的，而是可能由含 IgG 的免疫复合物诱导产生的。
