Sung K L, Dong C, Schmid-Schönbein G W, Chien S, Skalak R
Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Biophys J. 1988 Aug;54(2):331-6. doi: 10.1016/S0006-3495(88)82963-1.
Study of the mechanical properties of leukocytes is useful to understand their passage through narrow capillaries and interaction with other cells. Leukocytes are known to be viscoelastic and their properties have been established by micropipette aspiration techniques. Here, the recovery of leukocytes to their normal spherical form is studied after prolonged deformation in a pipette which is large enough to permit complete entry of the leukocyte. The recovery history is characterized by the time history of the major diameter (d1) and minor diameter (d2). When the cell is removed from the pipette, it shows initially a small rapid recoil followed by a slower asymptotic recovery to the spherical shape. In the presence of cell activation and formation of pseudopods, the time history for recovery is prolonged compared with passive cell recovery. If a protopod pre-existed during the holding period, the recovery only begins when the protopod starts to retract.
研究白细胞的力学特性有助于理解它们通过狭窄毛细血管的过程以及与其他细胞的相互作用。已知白细胞具有粘弹性，其特性已通过微量吸管抽吸技术得以确定。在此，研究了白细胞在足够大以允许其完全进入的吸管中长时间变形后恢复到正常球形的情况。恢复过程通过长轴直径（d1）和短轴直径（d2）的时间历程来表征。当细胞从吸管中取出时，它最初会有一个小的快速回缩，随后是较慢地渐近恢复到球形。在细胞活化和伪足形成的情况下，与被动细胞恢复相比，恢复的时间历程会延长。如果在保持期之前就存在原足，那么只有当原足开始回缩时恢复才会开始。
