Kim Hyeong-Dong, Cho Hyung-Rae, Moon Seung-Bae, Shin Hyun-Dong, Yang Kun-Ju, Park Bok-Ryeon, Jang Hee-Jeong, Kim Lin-Su, Lee Hyeung-Sik, Ku Sae-Kwang
Department of Physical Therapy, College of Health Science, Catholic University of Daegu 712-702, Korea.
Arch Pharm Res. 2007 Mar;30(3):323-8. doi: 10.1007/BF02977613.
The effects of beta-glucan isolated from Aureobasidium pullulans were observed on acute xylene-induced inflammation. beta-glucan at a dose of 62.5, 125 or 250 mg/kg were administered once orally to xylene-treated mice (0.03 mL of xylene was applied on the anterior surface of the right ear to induce inflammation), and the body weight change, ear weight, histological profiles and histomorphometrical analyses of ear were conducted upon sacrifice. The xylene was topically applied 30 min after dosing with beta-glucan. The results were compared to those of diclofenac, indomethacin and dexamethasone (15 mg/kg injected once intraperitoneally). All animals were sacrificed 2 h after xylene application. Xylene application resulted in marked increases in induced ear weights compared to that of intact control ear; hence, the differences between intact and induced ear were also significantly increased. The histological characteristics of acute inflammation, such as severe vasodilation, edematous changes of skin and infiltration of inflammatory cells, were detected in xylene-treated control ears with marked increase in the thickness of the ear tissues. However, these xylene-induced acute inflammatory changes were significantly and dose-dependently decreased by beta-glucan treatment. We conclude that beta-glucan from A. pullulans has a somewhat favorable effect in the reduction of the acute inflammatory responses induced by xylene application in mice.
观察了从出芽短梗霉中分离出的β-葡聚糖对二甲苯诱导的急性炎症的影响。将剂量为62.5、125或250mg/kg的β-葡聚糖经口一次性给予二甲苯处理的小鼠（在右耳前表面涂抹0.03mL二甲苯以诱导炎症），在处死后进行体重变化、耳重、组织学观察和耳部组织形态计量分析。在给予β-葡聚糖30分钟后局部涂抹二甲苯。将结果与双氯芬酸、吲哚美辛和地塞米松（15mg/kg腹腔注射一次）的结果进行比较。在涂抹二甲苯2小时后处死所有动物。与完整对照耳相比，涂抹二甲苯导致诱导耳重量显著增加；因此，完整耳与诱导耳之间的差异也显著增加。在二甲苯处理的对照耳中检测到急性炎症的组织学特征，如严重的血管扩张、皮肤水肿变化和炎性细胞浸润，耳部组织厚度显著增加。然而，β-葡聚糖处理可显著且剂量依赖性地减轻这些二甲苯诱导的急性炎症变化。我们得出结论，出芽短梗霉的β-葡聚糖在减轻小鼠二甲苯诱导的急性炎症反应方面具有一定的有益作用。
