Lee Hyeung-Sik, Cho Hyung-Rae, Yang Kun-Ju, Moon Seung-Bae, Park Bok-Ryeon, Shin Hyun-Dong, Jang Hee-Jeong, Kim Lin-Su, Ku Sae-Kwang
12Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan, 712-715 Korea.
Marine Biotechnology Center 221, Glucan Corp. Research Institute, Busan, 617-763 Korea.
Toxicol Res. 2008 Mar;24(1):11-15. doi: 10.5487/TR.2008.24.1.011. Epub 2008 Mar 1.
In this research the genotoxic effect of Polycan™ β-glucans originated from SM-2001, was evaluated using the mouse micronucleus test. Polycan™ was administered once a day for 2 days by oral gavage to male ICR mice at doses of 1000, 500 and 250 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control group. The appearance of a micronucleus is used as an index for genotoxic potential. The results obtained indicated that Polycan™ shows no genotoxicity effect up to 1000 mg/kg dosing levels. In addition, it is also considered that there were no problems from cytotoxicity of Polycan™ tested in this study because the polychromatic erythrocyte ratio was detected as > 0.47 in all tested groups.
在本研究中，使用小鼠微核试验评估了源自SM - 2001的聚多糖™β - 葡聚糖的遗传毒性作用。以1000、500和250 mg/kg的剂量，通过口服灌胃法，每天给雄性ICR小鼠施用聚多糖™，持续2天。环磷酰胺在阳性对照组中用作已知的遗传毒性剂。微核的出现用作遗传毒性潜力的指标。获得的结果表明，在高达1000 mg/kg的给药水平下，聚多糖™未显示出遗传毒性作用。此外，还认为在本研究中测试的聚多糖™的细胞毒性不存在问题，因为在所有测试组中检测到多染红细胞比率> 0.47。
