Conejo Nélida M, González Pardo Héctor, López Matías, Cantora Raúl, Arias Jorge L
Universidad de Oviedo.
Psicothema. 2007 May;19(2):295-301.
The involvement of the basolateral and the medial amygdala in fear conditioning was evaluated using different markers of neuronal activation. The method described here is a combination of cytochrome oxidase (CO) histochemistry and c-Fos immunocytochemistry on fresh frozen brain sections. Freezing behavior was used as an index of auditory and contextual fear conditioning. As expected, freezing scores were significantly higher in rats exposed to tone-shock pairings in a distinctive environment (conditioned; COND), as compared to rats that did not receive any shocks (UNCD). CO labeling was increased in the basolateral and medial amygdala of the COND group. Conversely, c-Fos expression in the basolateral and medial amygdala was lower in the COND group as compared to the UNCD group. Furthermore, c-Fos expression was particularly high in the medial amygdala of the UNCD group. The data provided by both techniques indicate that these amygdalar nuclei could play different roles on auditory and contextual fear conditioning.
利用不同的神经元激活标记物评估基底外侧杏仁核和内侧杏仁核在恐惧条件反射中的参与情况。这里描述的方法是对新鲜冷冻脑切片进行细胞色素氧化酶（CO）组织化学和c-Fos免疫细胞化学的结合。冻结行为被用作听觉和情境恐惧条件反射的指标。正如预期的那样，与未接受任何电击的大鼠（未条件化；UNCD）相比，在独特环境中接受音调-电击配对的大鼠（条件化；COND）的冻结分数显著更高。COND组基底外侧和内侧杏仁核中的CO标记增加。相反，与UNCD组相比，COND组基底外侧和内侧杏仁核中的c-Fos表达较低。此外，UNCD组内侧杏仁核中的c-Fos表达特别高。这两种技术提供的数据表明，这些杏仁核在听觉和情境恐惧条件反射中可能发挥不同的作用。
