Akao Y, Seto M, Takahashi T, Saito M, Utsumi K R, Nakazawa S, Ueda R
Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya, Japan.
Cancer Res. 1991 Dec 15;51(24):6708-11.
We previously demonstrated that the breakpoint of t(11;14)(q23;q32) in the RC-K8 B cell lymphoma cell line lies between CD3 and THY1/ETS1 on chromosome 11q23, and we cloned this region and named it the rck locus. Pulsed-field gel electrophoresis showed that the rck probe B (distal to the breakpoint) and the porphobilinogen deaminase (PBGD) probe detect the same germ line band and also the same rearranged band when DNA from RC-K8 cells was digested with NotI enzyme. Furthermore, Southern blot analysis with somatic cell hybrids showed that the PBGD gene moved to the 14q+chromosome, which confirmed PBGD to be more distal to the centromere than the rck locus. These data allowed us to construct the following order of genes: 11 cen-q23-CD3-rck-PBGD-THY1/ETS1. In this study, three infantile leukemia cell lines with t(11;19)(q23;p13) translocation were also analyzed by pulsed-field gel electrophoresis. CD3D probe detected the rearranged bands in DNA from two of them after digestion with NotI and SacII enzymes, demonstrating that the breakpoints of both cell lines were estimated to be within 360 kilobases of CD3D.
我们先前证明，RC-K8 B细胞淋巴瘤细胞系中t(11;14)(q23;q32)的断点位于11号染色体q23上的CD3和THY1/ETS1之间，我们克隆了该区域并将其命名为rck基因座。脉冲场凝胶电泳显示，当用NotI酶消化RC-K8细胞的DNA时，rck探针B（位于断点远端）和胆色素原脱氨酶（PBGD）探针检测到相同的种系条带以及相同的重排条带。此外，用体细胞杂种进行的Southern印迹分析表明，PBGD基因转移到了14q +染色体上，这证实了PBGD比rck基因座更远离着丝粒。这些数据使我们能够构建以下基因顺序：11cen-q23-CD3-rck-PBGD-THY1/ETS1。在本研究中，还通过脉冲场凝胶电泳分析了三个具有t(11;19)(q23;p13)易位的婴儿白血病细胞系。用NotI和SacII酶消化后，CD3D探针在其中两个细胞系的DNA中检测到重排条带，表明这两个细胞系的断点估计都在CD3D的360千碱基范围内。
