使用基于蛋白质特异性单克隆抗体的乳胶凝集试验对社区获得性败血症患者进行类鼻疽伯克霍尔德菌感染的快速诊断。
Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia.
作者信息
Ekpo Pattama, Rungpanich Utane, Pongsunk Supinya, Naigowit Pimjai, Petkanchanapong Vimon
机构信息
Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
出版信息
Clin Vaccine Immunol. 2007 Jun;14(6):811-2. doi: 10.1128/CVI.00011-07. Epub 2007 Apr 11.
Abstract
A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.
摘要
采用针对伯克霍尔德菌30 kDa蛋白的单克隆抗体的乳胶凝集试验，检测1139例社区获得性败血症患者血培养标本中的细菌。该试验的敏感性、特异性、阳性预测值和阴性预测值分别为96.75%、99.61%、96.75%和99.61%。
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