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Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia.使用基于蛋白质特异性单克隆抗体的乳胶凝集试验对社区获得性败血症患者进行类鼻疽伯克霍尔德菌感染的快速诊断。
Clin Vaccine Immunol. 2007 Jun;14(6):811-2. doi: 10.1128/CVI.00011-07. Epub 2007 Apr 11.
2
Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia.基于单克隆抗体的社区获得性败血症患者血培养液中伯克霍尔德菌的快速鉴定。
J Med Microbiol. 2000 Dec;49(12):1075-1078. doi: 10.1099/0022-1317-49-12-1075.
3
Rapid identification of Burkholderia pseudomallei in blood cultures by latex agglutination using lipopolysaccharide-specific monoclonal antibody.使用脂多糖特异性单克隆抗体通过乳胶凝集法快速鉴定血培养中的类鼻疽伯克霍尔德菌。
Am J Trop Med Hyg. 1999 Oct;61(4):658-62. doi: 10.4269/ajtmh.1999.61.658.
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Rapid identification of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay.通过协同凝集试验快速鉴定血培养上清液中的类鼻疽伯克霍尔德菌。
Clin Microbiol Infect. 2005 Nov;11(11):930-3. doi: 10.1111/j.1469-0691.2005.01235.x.
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Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis.简短报告：基于单克隆抗体的乳胶凝集试验用于快速诊断败血症型类鼻疽的评估
Am J Trop Med Hyg. 1999 Nov;61(5):735-7. doi: 10.4269/ajtmh.1999.61.735.
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Rapid identification of Burkholderia pseudomallei in blood cultures by a monoclonal antibody assay.通过单克隆抗体检测法快速鉴定血培养中的类鼻疽伯克霍尔德菌。
J Clin Microbiol. 1999 Nov;37(11):3662-7. doi: 10.1128/JCM.37.11.3662-3667.1999.
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Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei.基于多糖的乳胶凝集试验用于快速检测伯克霍尔德菌抗体的评估
Am J Trop Med Hyg. 2015 Sep;93(3):542-546. doi: 10.4269/ajtmh.15-0114. Epub 2015 Jun 29.
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Rapid identification of Burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody.基于一种胞外多糖特异性单克隆抗体，通过乳胶凝集法快速鉴定类鼻疽伯克霍尔德菌。
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Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.一种乳胶凝集检测法用于鉴定鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的评价。
Am J Trop Med Hyg. 2014 Jun;90(6):1043-6. doi: 10.4269/ajtmh.14-0025. Epub 2014 Apr 7.
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Cross-reactivity of latex agglutination assay complicates the identification of Burkholderia pseudomallei from soil.乳胶凝集检测的交叉反应使得从土壤中鉴定类鼻疽伯克霍尔德菌变得复杂。
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Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay.类鼻疽快速诊断：新型侧向流动抗原检测试验的比较研究
J Med Microbiol. 2015 Aug;64(8):845-848. doi: 10.1099/jmm.0.000098. Epub 2015 Jun 7.
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Melioidosis diagnostic workshop, 2013.类鼻疽诊断研讨会，2013年。
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本文引用的文献

1
Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes.使用荧光杂交探针通过实时聚合酶链反应检测法快速初步鉴定类鼻疽伯克霍尔德菌。
Mol Cell Probes. 2005 Feb;19(1):9-20. doi: 10.1016/j.mcp.2004.08.001.
2
Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.通过靶向III型分泌系统基因的实时PCR鉴定和区分伯克霍尔德菌、鼻疽伯克霍尔德菌和泰国伯克霍尔德菌
J Clin Microbiol. 2004 Dec;42(12):5871-4. doi: 10.1128/JCM.42.12.5871-5874.2004.
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Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex.用于快速鉴定鼻疽伯克霍尔德菌//类鼻疽伯克霍尔德菌复合体的5'核酸酶实时荧光定量PCR检测方法的建立
Diagn Mol Pathol. 2004 Dec;13(4):247-53. doi: 10.1097/01.pdm.0000137099.36618.cc.
4
Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei.使用16S rRNA基因测序快速鉴定和区分类鼻疽伯克霍尔德菌和鼻疽伯克霍尔德菌。
J Clin Microbiol. 2003 Oct;41(10):4647-54. doi: 10.1128/JCM.41.10.4647-4654.2003.
5
Ubiquity of putative type III secretion genes among clinical and environmental Burkholderia pseudomallei isolates in Northern Australia.澳大利亚北部临床和环境类鼻疽伯克霍尔德菌分离株中假定的III型分泌基因的普遍性。
J Clin Microbiol. 2003 Feb;41(2):883-5. doi: 10.1128/JCM.41.2.883-885.2003.
6
Single gene target bacterial identification. groEL gene sequencing for discriminating clinical isolates of Burkholderia pseudomallei and Burkholderia thailandensis.单基因靶向细菌鉴定。用于区分伯克霍尔德菌属假鼻疽亚种和泰国伯克霍尔德菌临床分离株的groEL基因测序。
Diagn Microbiol Infect Dis. 2002 Oct;44(2):143-9. doi: 10.1016/s0732-8893(02)00439-x.
7
A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene.在使用基于鞭毛蛋白fliC基因序列的分子鉴定系统鉴定鼻疽伯克霍尔德菌时可能存在的一个陷阱。
FEMS Immunol Med Microbiol. 2002 Nov 15;34(3):231-6. doi: 10.1111/j.1574-695X.2002.tb00629.x.
8
Identification of a novel repetitive DNA element and its use as a molecular marker for strain typing and discrimination of ara- from ara+ Burkholderia pseudomallei isolates.一种新型重复DNA元件的鉴定及其作为分子标记用于菌株分型和区分ara-型与ara+型类鼻疽伯克霍尔德菌分离株的应用。
J Med Microbiol. 2002 Jan;51(1):76-82. doi: 10.1099/0022-1317-51-1-76.
9
Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia.基于单克隆抗体的社区获得性败血症患者血培养液中伯克霍尔德菌的快速鉴定。
J Med Microbiol. 2000 Dec;49(12):1075-1078. doi: 10.1099/0022-1317-49-12-1075.
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Recent developments in laboratory diagnosis of melioidosis.类鼻疽实验室诊断的最新进展。
Acta Trop. 2000 Feb 5;74(2-3):235-45. doi: 10.1016/s0001-706x(99)00076-5.

使用基于蛋白质特异性单克隆抗体的乳胶凝集试验对社区获得性败血症患者进行类鼻疽伯克霍尔德菌感染的快速诊断。

Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia.

作者信息

Ekpo Pattama, Rungpanich Utane, Pongsunk Supinya, Naigowit Pimjai, Petkanchanapong Vimon

机构信息

Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

出版信息

Clin Vaccine Immunol. 2007 Jun;14(6):811-2. doi: 10.1128/CVI.00011-07. Epub 2007 Apr 11.

DOI:10.1128/CVI.00011-07
PMID:17428952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1951097/
Abstract

A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.

摘要

采用针对伯克霍尔德菌30 kDa蛋白的单克隆抗体的乳胶凝集试验，检测1139例社区获得性败血症患者血培养标本中的细菌。该试验的敏感性、特异性、阳性预测值和阴性预测值分别为96.75%、99.61%、96.75%和99.61%。