Lee R J, Mackenzie I C, Robinson B K, Gantz B J
Ear, Nose and Throat Department, Kettering General Hospital, UK.
Clin Otolaryngol Allied Sci. 1991 Oct;16(5):493-7. doi: 10.1111/j.1365-2273.1991.tb01047.x.
The exact nature and role of the epithelial layer in cholesteatoma remains undetermined. The aim of this study was to investigate cholesteatoma epithelium and normal aural epithelia in common cell culture conditions. Samples of cholesteatoma, external meatal epidermis and middle ear mucosa were obtained, successfully grown in cell culture, and subcultured. No significant morphological differences were found between cholesteatoma and aural epidermis. The only differences noted were delayed onset of colony formation, and the need to subculture prior to the cultures becoming confluent in the cholesteatoma cultures. Further research is required to account for these differences in growth patterns.
上皮层在胆脂瘤中的确切性质和作用仍未确定。本研究的目的是在共同的细胞培养条件下研究胆脂瘤上皮和正常耳上皮。获取胆脂瘤、外耳道表皮和中耳黏膜样本，成功在细胞培养中生长并传代培养。胆脂瘤和耳表皮之间未发现明显的形态学差异。唯一注意到的差异是集落形成起始延迟，以及胆脂瘤培养物在汇合前需要传代培养。需要进一步研究来解释这些生长模式上的差异。
