Tanomaru-Filho Mário, Tanomaru Juliane M G, Barros Danilo B, Watanabe Evandro, Ito Izabel Y
Restorative Dentistry, Araraquara Dental School, São Paulo State University, Araraquara, SP, Brazil.
J Oral Sci. 2007 Mar;49(1):41-5. doi: 10.2334/josnusd.49.41.
The aim of this study was to evaluate the antimicrobial activity of different root-end filling materials - Sealer 26, Sealapex with zinc oxide, zinc oxide and eugenol, white and gray Portland cement, white and gray MTA-Angelus, and gray Pro Root MTA - against six different microorganism strains. The agar diffusion method was used. A base layer was made using Müller-Hinton agar (MH) and wells were formed by removing the agar. The materials were placed in the wells immediately after manipulation. The microorganisms used were: Micrococcus luteus (ATCC9341), Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC10538), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC 10231), and Enterococcus faecalis (ATCC 10541). The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37 degrees C for 24 h. Triphenyltetrazolium chloride 0.05% gel was added for optimization, and the zones of inhibition were measured. Data were subjected to the Kruskal-Wallis and Dunn tests at a 5% significance level. The results showed that all materials had antimicrobial activity against all the tested strains. Analysis of the efficacy of the materials against the microbial strains showed that Sealapex with zinc oxide, zinc oxide and eugenol and Sealer 26 created larger inhibition halos than the MTA-based and Portland cements (P < 0.05). On the basis of the methodology used, it may be concluded that all endodontic sealers, MTA-based and Portland cements evaluated in this study possess antimicrobial activity, particularly the endodontic sealers.
本研究旨在评估不同根尖充填材料——Sealer 26、含氧化锌的Sealapex、氧化锌丁香油糊剂、白色和灰色波特兰水泥、白色和灰色MTA - Angelus以及灰色Pro Root MTA——对六种不同微生物菌株的抗菌活性。采用琼脂扩散法。使用Müller - Hinton琼脂（MH）制作基层，通过去除琼脂形成孔。材料在操作后立即放入孔中。所使用的微生物为：藤黄微球菌（ATCC9341）、金黄色葡萄球菌（ATCC6538）、大肠埃希菌（ATCC10538）、铜绿假单胞菌（ATCC27853）、白色念珠菌（ATCC 10231）和粪肠球菌（ATCC 10541）。平板在室温下预扩散2小时，然后在37℃下孵育24小时。加入0.05%的氯化三苯基四氮唑凝胶进行优化，并测量抑菌圈。数据在5%显著性水平下进行Kruskal - Wallis和Dunn检验。结果表明，所有材料对所有测试菌株均具有抗菌活性。对材料针对微生物菌株的疗效分析表明，含氧化锌的Sealapex、氧化锌丁香油糊剂和Sealer 26比基于MTA的材料和波特兰水泥产生更大的抑菌圈（P < 0.05）。基于所使用的方法，可以得出结论，本研究中评估的所有根管封闭剂，包括基于MTA的材料和波特兰水泥，均具有抗菌活性，尤其是根管封闭剂。
