Hasan Zarrabi Mohammad, Javidi Maryam, Naderinasab Mahboube, Gharechahi Maryam
Department of Endodontics, Faculty of Dentistry and Dental Research Centre, Mashhad University of Medical Sciences, Iran.
J Oral Sci. 2009 Sep;51(3):437-42. doi: 10.2334/josnusd.51.437.
Using the agar diffusion method, we conducted an in vitro study to evaluate the antimicrobial activity of mineral trioxide aggregate (MTA), new endodontic cement (NEC) and Portland cement at different concentrations against five different microorganisms. A base layer was made using Muller-Hinton agar for Escherichia coli (ATCC 10538) and Candida (ATCC 10231). For Actinomyces viscosus (ATCC 15987), Enterococcus faecalis (ATCC 10541) and Streptococcus mutans (ATCC 25175) blood agar medium was used. Wells were formed by removing the agar, and the materials were placed in the well immediately after manipulation. The plates were kept at room temperature for 2 h for prediffusion, and then incubated at 37 degrees C for 72 h. The inhibition zones were then measured. The data were analyzed using ANOVA and the Tukey test to compare the differences among the three cements at different concentrations. The positive controls showed bacterial growth, while the negative controls showed no bacterial growth. All materials showed antimicrobial activity against the tested strains except for Enterococcus faecalis. NEC created larger inhibition zones than MTA and Portland cement. This difference was significant for Portland cement (P < 0.05), but not for MTA (P > 0.05). Among the examined microorganisms, the largest inhibition zone was observed for Actinomyces group (P < 0.05). The antimicrobial activity of the materials increased with time and concentration (P < 0.05). It was concluded that NEC is a potent inhibitor of microorganism growth.
我们采用琼脂扩散法进行了一项体外研究，以评估不同浓度的三氧化矿物凝聚体（MTA）、新型根管充填水门汀（NEC）和波特兰水泥对五种不同微生物的抗菌活性。对于大肠杆菌（ATCC 10538）和白色念珠菌（ATCC 10231），使用Muller-Hinton琼脂制作基层。对于粘性放线菌（ATCC 15987）、粪肠球菌（ATCC 10541）和变形链球菌（ATCC 25175），则使用血琼脂培养基。通过移除琼脂形成孔，材料在操作后立即放入孔中。平板在室温下放置2小时进行预扩散，然后在37℃下孵育72小时。随后测量抑菌圈。使用方差分析（ANOVA）和Tukey检验分析数据，以比较三种水门汀在不同浓度下的差异。阳性对照显示有细菌生长，而阴性对照未显示细菌生长。除粪肠球菌外，所有材料对受试菌株均显示出抗菌活性。NEC产生的抑菌圈比MTA和波特兰水泥更大。波特兰水泥的这种差异具有统计学意义（P < 0.05），而MTA则无统计学意义（P > 0.05）。在所检测的微生物中，放线菌组观察到的抑菌圈最大（P < 0.05）。材料的抗菌活性随时间和浓度增加（P < 0.05）。研究得出结论，NEC是一种有效的微生物生长抑制剂。
