Leonardo M R, da Silva L A, Tanomaru Filho M, Bonifácio K C, Ito I Y
Departamento de Endodontia, Faculdade de Odontologia de Araraquara-UNESP, Caixa Postal 331, Rua Humaitá, 1680, 14801-903 Araraquara, SP, Brazil.
J Endod. 2000 Jul;26(7):391-4. doi: 10.1097/00004770-200007000-00003.
The antimicrobial activity of four root canal sealers (AH Plus, Sealapex, Ketac Endo, and Fill Canal), two calcium hydroxide pastes (Calen and Calasept), and a zinc oxide paste was evaluated. Seven bacterial strains were used, six of them standard; Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, and Enterococcus faecalis ATCC 10541. There was a wild strain of Streptococcus mutans isolated from saliva obtained in an adult dental clinic. Activity was evaluated using the agar diffusion method with Brain Heart Infusion agar and Müller Hinton medium seeded by pour plate. Calcium hydroxide-based sealers and pastes were either placed directly into 4.0 x 4.0 mm wells or by using absorbent paper points. The plates were kept at room temperature for 2 hr for diffusion. After incubation at 37 degrees C for 24 hr, the medium was optimized with 0.05 g% TTC gel and inhibition haloes were measured. All bacterial strains were inhibited by all materials using the well method. However, when the materials were applied with absorbent paper points, Enterococcus faecalis was not inhibited by zinc oxide, and Pseudomonas aeruginosa was not inhibited by AH Plus, Fill Canal, and the zinc oxide-based paste. We conclude that sealers and pastes presented antimicrobial activity in vitro and culture medium optimization with 0.05 g% TTC gel facilitated observation of the inhibition haloes.
评估了四种根管封闭剂（AH Plus、Sealapex、Ketac Endo和Fill Canal）、两种氢氧化钙糊剂（Calen和Calasept）以及一种氧化锌糊剂的抗菌活性。使用了七种细菌菌株，其中六种为标准菌株：藤黄微球菌ATCC 9341、金黄色葡萄球菌ATCC 25923、铜绿假单胞菌ATCC 27853、表皮葡萄球菌ATCC 12228、大肠埃希菌ATCC 25922和粪肠球菌ATCC 10541。还有一种从成人牙科诊所采集的唾液中分离出的变形链球菌野生菌株。采用琼脂扩散法，使用脑心浸液琼脂和倾注平板接种的Müller Hinton培养基评估活性。基于氢氧化钙的封闭剂和糊剂要么直接放入4.0×4.0毫米的孔中，要么使用吸水纸尖放置。平板在室温下放置2小时以进行扩散。在37℃孵育24小时后，用0.05 g%的TTC凝胶优化培养基并测量抑菌圈。使用孔法时，所有材料都能抑制所有细菌菌株。然而，当使用吸水纸尖应用这些材料时，氧化锌不能抑制粪肠球菌，AH Plus、Fill Canal和氧化锌基糊剂不能抑制铜绿假单胞菌。我们得出结论，封闭剂和糊剂在体外呈现抗菌活性，用0.05 g%的TTC凝胶优化培养基有助于观察抑菌圈。
