Liu Yunying, Zheng Wei, Li Liwei, Mao Yafei, Yan Jie
Department of Medical Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Med Microbiol Immunol. 2007 Dec;196(4):233-9. doi: 10.1007/s00430-007-0047-0. Epub 2007 Apr 12.
Interactions of virulent Leptospira interrogans with murine monocyte-macrophage-like J774A.1 cells and Vero (African green monkey kidney fibroblasts) cells from attachment to internalization were investigated by a series of morphological analysis. Fontana silver staining revealed that only the pathogenic leptospires were able to attach to host cells and the attachment pattern varied depending on cell types that they interacted with. Transmission electron microscopy (TEM) analysis confirmed the formation of the leptospires-containing phagosomes and their colocalization with lysosomes in macrophages were verified by confocal microscopic analysis. Results of F-actin rearrangements examination indicated that virulent leptospires invaded host cells via a microfilament-independent pathway.
通过一系列形态学分析，研究了致病性问号钩端螺旋体与小鼠单核细胞-巨噬细胞样J774A.1细胞以及非洲绿猴肾成纤维细胞Vero从附着到内化的相互作用。Fontana银染色显示，只有致病性钩端螺旋体能够附着于宿主细胞，且附着模式因与之相互作用的细胞类型而异。透射电子显微镜（TEM）分析证实了含钩端螺旋体吞噬体的形成，共聚焦显微镜分析验证了其在巨噬细胞中与溶酶体的共定位。F-肌动蛋白重排检测结果表明，致病性钩端螺旋体通过非微丝依赖途径侵入宿主细胞。
