锌和pH值对暴露于铅的狒狒体内δ-氨基乙酰丙酸脱水酶活性行为的影响。
The effect of zinc and pH on the behaviour of delta-aminolevulinic acid dehydratase activity in baboons exposed to lead.
作者信息
Cantrell A C, Kilroe-Smith T A, Simões M M, Border E A
出版信息
Br J Ind Med. 1977 May;34(2):110-3. doi: 10.1136/oem.34.2.110.
Four adult male baboons (Papio ursinus) were exposed to a cloud of lead oxide dust to induce changes in the status of delta-aminolevulinic acid dehydratase (ALAD). Enzyme activity fell rapidly to a steady state as blood lead levels rose above normal. Exogenous zinc was shown to activate the enzyme, and the antagonistic effect of zinc on in vivo and in vitro lead inhibition was demonstrated for baboons. In baboons not exposed to lead dust, ALAD showed an activity optimum at pH 7-1 which shifted to pH 6-8 with in vitro addition of lead. In baboons exposed to lead dust, with raised blood lead, activity optima were observed at pH 6-8 and 6-2, while the optimum at pH 7-1 was absent.
将四只成年雄性狒狒（山魈）暴露于氧化铅粉尘中，以诱导δ-氨基乙酰丙酸脱水酶（ALAD）状态发生变化。随着血铅水平升至正常水平以上，酶活性迅速降至稳定状态。外源性锌被证明可激活该酶，并且在狒狒体内和体外均证实了锌对铅抑制作用的拮抗效应。在未暴露于铅尘的狒狒中，ALAD在pH 7-1时表现出最佳活性，在体外添加铅后该最佳活性转移至pH 6-8。在暴露于铅尘的狒狒中，随着血铅升高，在pH 6-8和6-2观察到最佳活性，而在pH 7-1时的最佳活性消失。
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