Kulinsky V I, Kolesnichenko L S
Vopr Med Khim. 1975 May-Jun;21(3):286-90.
An increase in activity of disulphide reductase system (DRS) in supernatant of liver tissue was caused by 3',5'-AMP, ATP, GTP, UTP, Mg2+, Ca2+, EDTA, protamine, noradrenaline and F-. The effect was connected with arsenite resistant fraction of DRS. After rapid homogenization the effect of noradrenaline disappeared and the effects of ATP, GTP, UTP and Ca2+ were distinctly decreased. Treatment with adsorbents prevented the effects of 3',5,-AMP, ATP and EDTA and markedly decreased the effects of protamine and Mg2+. A protein inhibitor of protein kinase prevented completely the activation of DRS with 3',5'-AMP, ATP, GTP, UTP and noradrenaline and distinctly decreased the effect of protamine, Mg2+ and Ca2+ but did not alter the influence of EDTA. Addition of 2',3'-AMP blocked the effect of 3',5-AMP, ATP and Mg2+ but did not influence the effect of protamine and EDTA. The data obtained suggest that protein kinase participated in activation of DRS by most of the regulators.
3',5'-AMP、ATP、GTP、UTP、Mg2+、Ca2+、EDTA、鱼精蛋白、去甲肾上腺素和F-可导致肝组织上清液中二硫键还原酶系统（DRS）活性增加。该作用与DRS的抗亚砷酸盐部分有关。快速匀浆后，去甲肾上腺素的作用消失，ATP、GTP、UTP和Ca2+的作用明显减弱。用吸附剂处理可阻止3',5,-AMP、ATP和EDTA的作用，并显著降低鱼精蛋白和Mg2+的作用。蛋白激酶的蛋白抑制剂可完全阻止3',5'-AMP、ATP、GTP、UTP和去甲肾上腺素对DRS的激活，并显著降低鱼精蛋白、Mg2+和Ca2+的作用，但不改变EDTA的影响。添加2',3'-AMP可阻断3',5-AMP、ATP和Mg2+的作用，但不影响鱼精蛋白和EDTA的作用。所获得的数据表明，蛋白激酶参与了大多数调节剂对DRS的激活。
