Kulinskii V I, Kolesnichenko L S, Trufanova L V
Biokhimiia. 1977 Aug;42(8):1399-1407.
The activation of 2 different mouse liver enzymes: cytozolic disulfide reductase (DSR) and mitochondrial NAD-isocitrate dehydrogenase (ICDH), by catecholamines and especially by 3',5'-AMP is characterized by negative cooperativity; substrate (both enzymes), protamine and EDTA (DSR) produce the positive cooperativity type of activation; DSR activation by isopropyl noradrenaline and serotonine is characterized by hyperbolic kinetics. Consequently, one and the same enzyme can combine positive cooperativity to non-specialized regulators (substrate, protamine, EDTA) with negative cooperativity to specialized regulators (3',5'-AMP, catecholamines). The systems, switching on by catecholamines and 3',5'-AMP, are oligomeric, and the degree and even the type of cooperativity can modify depending on the kind of catecholamine. The negative cooperativity is revealed in literature for many effects of catecholamines and 3',5'-AMP. Probably, it guarantees the broad range of regulations. Dose effect curves for 3',5'-AMP, catecholamines and other hormones should be analyzed on the basis of allosteric protein kinetics. A simple nomogram is given to estimate nH less than 1.
儿茶酚胺，尤其是3',5'-AMP对两种不同的小鼠肝脏酶：胞质二硫化物还原酶（DSR）和线粒体NAD-异柠檬酸脱氢酶（ICDH）的激活具有负协同性；底物（两种酶）、鱼精蛋白和EDTA（DSR）产生正协同性类型的激活；异丙去甲肾上腺素和血清素对DSR的激活具有双曲线动力学特征。因此，同一种酶可以将对非特异性调节剂（底物、鱼精蛋白、EDTA）的正协同性与对特异性调节剂（3',5'-AMP、儿茶酚胺）的负协同性结合起来。由儿茶酚胺和3',5'-AMP开启的系统是寡聚体，协同性的程度甚至类型会根据儿茶酚胺的种类而改变。文献中揭示了儿茶酚胺和3',5'-AMP的许多效应存在负协同性。可能，这保证了广泛的调节范围。3',5'-AMP、儿茶酚胺和其他激素的剂量效应曲线应基于别构蛋白动力学进行分析。给出了一个简单的列线图来估计nH小于1。
