Renesto P G, Ponciano V C, Cenedeze M A, Saraiva Câmara N O, Pacheco-Silva A
Laboratory of Clinical and Experimental Immunology, Division of Nephrology, Universidade Federal de São Paulo, São Paulo, Brazil.
Am J Transplant. 2007 Jun;7(6):1661-5. doi: 10.1111/j.1600-6143.2007.01795.x. Epub 2007 Apr 8.
TIM-3 is a recently described molecule specifically expressed on Th1 differentiated T cells. We explored the usefulness of urinary mRNA profiles in the diagnosis of renal acute rejection (AR). Sixty urinary samples from renal transplant recipients simultaneously collected to allograft biopsy, (AR = 30 and No-AR =30), and 12 urinary samples from stable renal transplants were analyzed. Urinary mRNA encoding for TIM-3 and IFN-gamma were quantified using real time RT-PCR. TIM-3 mRNA was highly expressed in AR (559.19 +/- 644.41) compared to No-AR (3.78 +/- 7.20), and stable transplants (0.54 +/- 0.76), p < 0.001. To a lesser degree, IFN-gamma mRNA transcripts were also increased in AR (50.40 +/- 38.71), compared with No-AR (4.69 +/- 12.62), and stable transplants (0.38 +/- 0.44) p < 0.001. The highest expression of TIM-3 in AR makes it a promising noninvasive test for its diagnosis.
TIM-3是一种最近被描述的分子，在Th1分化的T细胞上特异性表达。我们探讨了尿mRNA谱在肾急性排斥反应（AR）诊断中的作用。同时收集了60份来自肾移植受者的尿样并进行同种异体移植活检（AR组30例，非AR组30例），并分析了12份来自稳定肾移植受者的尿样。使用实时逆转录聚合酶链反应对编码TIM-3和干扰素-γ的尿mRNA进行定量。与非AR组（3.78±7.20）和稳定移植组（0.54±0.76）相比，TIM-3 mRNA在AR组中高表达（559.19±644.41），p<0.001。与非AR组（4.69±12.62）和稳定移植组（0.38±0.44）相比，干扰素-γ mRNA转录本在AR组中也有较小程度的增加，p<0.001。TIM-3在AR中的最高表达使其成为一种有前景的无创诊断检测方法。
