Abouzed Tarek Kamal, Althobaiti Fayez, Omran Alaa Faik, Eldomany Ehab Bedir, El-Shazly Samir Ahmed, Alharthi Fahad, Elkattawy Azza Mansour, Kahilo Khaled Abd Aleim, Dorghamm Doaa Abdallha
Biochemistry Department, Faculty of Veterinary, Medicine Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
Biotechnology Department, College of Science, Taif University, Taif 21995, Saudi Arabia.
Toxicol Res (Camb). 2021 Dec 4;11(1):22-31. doi: 10.1093/toxres/tfab118. eCollection 2022 Feb.
Natural antioxidant products play a vital role in the treatment and prevention of cancer disease because they have no side effects. This study aimed to compare the chemoprotective effect of Spirulina platensis (SP) and garlic against hepatocellular carcinoma (HCC) in rats. This study was being done by using 60 male Wistar rats and divided into four groups. Group (I): normal group. Group (II): HCC group induced by injection of a single dose of DEN (200 mg/kg/I.P) and after 14 days injected CCl4 (1 mg/kg/I.P) 3 times/week/six weeks. Group (III): HCC group received SP orally at a dose (500 mg/kg). Group (IV): HCC group received garlic (250 mg/kg) orally. The results revealed that the Spirulina and garlic treatment have a significant decrease in Glutamate pyruvate transaminase, Glutamate oxaloacetate transaminase, GGT, LDH, and the Malondialdehyde (MDA) activity, and furthermore, a significant increase in the total protein level, the superoxide dismutase (SOD), and Catalase (CAT) activity nearly to normal activity. Furthermore, the hepatic expression of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), inducible nitric oxide synthase, transforming growth factor-beta (TGF-β1), Heat Shock Protein glycoprotein 96 (HSPgp96), and Glypican 3 (GP3) were down regulated by the Spirulina and garlic treatment in comparison with those in HCC group. All findings reported that the chemoprotective of both Spirulina and garlic that have nearly the same effect may be due to antioxidant activity and inhibition of lipid peroxidation, amelioration of pro-inflammatory cytokine, HSPgp96, and GP3.
天然抗氧化剂产品在癌症疾病的治疗和预防中起着至关重要的作用，因为它们没有副作用。本研究旨在比较钝顶螺旋藻（SP）和大蒜对大鼠肝细胞癌（HCC）的化学保护作用。本研究使用60只雄性Wistar大鼠，分为四组。第一组：正常组。第二组：通过单次注射二乙基亚硝胺（DEN，200mg/kg，腹腔注射）诱导的HCC组，14天后每周3次、连续6周注射四氯化碳（CCl4，1mg/kg，腹腔注射）。第三组：HCC组口服SP，剂量为500mg/kg。第四组：HCC组口服大蒜（250mg/kg）。结果显示，螺旋藻和大蒜治疗后，谷丙转氨酶、谷草转氨酶、γ-谷氨酰转肽酶、乳酸脱氢酶和丙二醛（MDA）活性显著降低，此外，总蛋白水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著增加，几乎恢复到正常活性。此外，与HCC组相比，螺旋藻和大蒜治疗下调了肿瘤坏死因子（TNF-α）、白细胞介素-6（IL-6）、诱导型一氧化氮合酶、转化生长因子-β1（TGF-β1）、热休克蛋白糖蛋白96（HSPgp96）和磷脂酰肌醇蛋白聚糖3（GP3）的肝脏表达。所有研究结果表明，螺旋藻和大蒜具有几乎相同效果的化学保护作用可能归因于抗氧化活性、对脂质过氧化的抑制、促炎细胞因子、HSPgp96和GP3的改善。
