Tumor Research and Therapy Center, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, People's Republic of China.
Department of Pain Management, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China.
Int J Nanomedicine. 2020 Oct 27;15:8249-8260. doi: 10.2147/IJN.S272997. eCollection 2020.
Lung cancer is one of the most aggressive forms of cancer that leads to a high mortality rate amongst several cancer types and it is a widely recurrent cancer globally. The use of zinc oxide nanoparticles (ZnONPs) in the formulation of sun cream, food flavors, and colorings due to its varied biological properties. The extensive significance of nanoparticles encourages their production but the approaches are a common challenge in concluding the direct beneficial effect for the disease treatment. Hence, in the present study, zinc oxide-loaded syringic acid (ZnO-SYR) phytochemical was used to elucidate the therapeutic effect against lung cancer.
The ZnO-SYR nanoparticles were synthesized and characterized by UV-visible spectroscopy, X-ray diffraction, dynamic light scattering, and FT-IR analysis. The characterized ZnO-SYR was tested on in vivo mouse model of lung cancer (benzo(a)pyrene (BAP)) and in vitro A549 cells.
The results demonstrated the significant restoration of body weight with attenuated serum marker enzymes compared to BAP-treated animals. In addition, cytokine estimation revealed ameliorated levels of TNF-α, interleukins, IL-6, IL-1β with evidenced histological observations in ZnO-SYR-treated mice compared to BAP-induced lung cancer mice.
Furthermore, cytotoxicity analysis demonstrated the altered mitochondrial membrane potential (MMP), with a profound increase in reactive oxygen species (ROS) levels, and apoptosis mechanism by ZnO-SYR compared to control cells. The conclusions of the present study put forward an evident confirmation of the protective and beneficial effects of zincoxide-loaded syringic acid against the BAP-induced lung cancer model.
肺癌是最具侵袭性的癌症之一，导致几种癌症类型的死亡率较高，并且是全球广泛复发的癌症。氧化锌纳米粒子（ZnONPs）由于其多种生物学特性，被广泛应用于防晒霜、食品香料和着色剂的配方中。由于纳米粒子具有广泛的重要性，鼓励其生产，但这些方法是在得出直接有益于疾病治疗的效果方面的常见挑战。因此，在本研究中，使用负载氧化锌的丁香酸（ZnO-SYR）植物化学物质来阐明其对肺癌的治疗效果。
通过紫外-可见光谱、X 射线衍射、动态光散射和傅里叶变换红外（FT-IR）分析对 ZnO-SYR 纳米粒子进行合成和表征。对体内肺癌（苯并（a）芘（BAP））小鼠模型和体外 A549 细胞进行了表征的 ZnO-SYR 测试。
结果表明，与 BAP 处理的动物相比，体重显著恢复，血清标志物酶水平降低。此外，与 BAP 诱导的肺癌小鼠相比，细胞因子估计显示 TNF-α、白细胞介素、IL-6 和 IL-1β水平得到改善，并在 ZnO-SYR 处理的小鼠中得到了明显的组织学观察。
此外，与对照细胞相比，细胞毒性分析表明，ZnO-SYR 改变了线粒体膜电位（MMP），活性氧（ROS）水平显著增加，并通过凋亡机制发挥作用。本研究的结论提出了负载氧化锌的丁香酸对 BAP 诱导的肺癌模型具有保护和有益作用的明显证据。
