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[超临界二氧化碳萃取鲜鱼腥草挥发油的正交试验及挥发油的气相色谱-质谱联用分析]

[Orthogonal experiment using SFE-CO2 in extraction of essential oil from fresh Houttuynia cordata and analysis of essential oil by GC-MS].

作者信息

Meng Jiang, Dong Xiao-ping, Zhou Yi-sheng, Jiang Zhi-hong, Leung Kelvin Sze-Yin, Zhao Zhong-zhen

机构信息

School of Chinese Medicine of Hong Kong Baptist University, Hong Kong, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):215-7.

PMID:17432141
Abstract

OBJECTIVE

To optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil.

METHOD

The extraction procedure of essential oil from fresh H. cordata was optimized with the orthogonal experiment. Essential oil of fresh H. cordata was analysed by GC-MS.

RESULT

The optimize preparative procedure was as follow: essential oil of H. cordata was extracted at a temperature of 35 degrees C, pressure of 15,000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified.

CONCLUSION

The optimum preparative procedure is reliable and can guarantee the quality of essential oil.

摘要

目的

优化超临界流体萃取-二氧化碳（SFE-CO2）法从蕺菜中提取挥发油的工艺，并分析挥发油的化学成分。

方法

采用正交试验对新鲜蕺菜挥发油的提取工艺进行优化。采用气相色谱-质谱联用（GC-MS）法分析新鲜蕺菜的挥发油。

结果

优化后的制备工艺如下：在35℃、15000 kPa压力下提取蕺菜挥发油20分钟。鉴定出38种化学成分并对其相对含量进行了定量分析。

结论

该优化制备工艺可靠，可保证挥发油的质量。

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