Yuan Zheng-zhong, Zhu Ling-qun, Pang He, Shan Ze-song, Wang Shuo-ren, Gao Yong-hong, Niu Fu-ling
First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):249-52.
To investigate the protective effect of geniposide, baicalin and berberine for the rat cerebral microvascular endothelial cell.
The model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established. Both normal and model cells were treated with geniposide (1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008 micromol x mL(-1)), baicalin (0.224, 0.112, 0.056, 0.028, 0.014, 0.007, 0.003 micromol x mL(-1)) and berberine (0.192, 0.096, 0.048, 0.024, 0.012, 0.006, 0.003 micromol x mL(-1)). Cell activity was measured by methyl thiazolyl tetrazolium (MTT) test.
After hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour, geniposide (0.128, 0.064, 0.032 micromol x mL(-1)), baicalin (0.028, 0.014, 0.007 micromol x mL(-1)) and berberine (0.024, 0.012, 0.006 micromol x microL(-1) could protect the injuried cerebral microvascular endothelial cells.
Appropriate concentration of geniposide, baicalin and berberine, which are effective components of Huanglian Jiedu decoction, could protect the injuried cerebral microvascular endothelial cells.
探讨栀子苷、黄芩苷和小檗碱对大鼠脑微血管内皮细胞的保护作用。
建立体外大鼠脑微血管内皮细胞缺氧复氧损伤模型。正常细胞和模型细胞分别用栀子苷（1.024、0.512、0.256、0.128、0.064、0.032、0.016、0.008 μmol·mL⁻¹）、黄芩苷（0.224、0.112、0.056、0.028、0.014、0.007、0.003 μmol·mL⁻¹）和小檗碱（0.192、0.096、0.048、0.024、0.012、0.006、0.003 μmol·mL⁻¹）处理。采用噻唑蓝（MTT）法检测细胞活性。
缺氧/低糖培养4小时并复氧12小时后，栀子苷（0.128、0.064、0.032 μmol·mL⁻¹）、黄芩苷（0.
