[黄连解毒汤有效成分对大鼠脑微血管内皮细胞缺氧复氧损伤的保护作用]

[Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells].

作者信息

Yuan Zheng-zhong, Zhu Ling-qun, Pang He, Shan Ze-song, Wang Shuo-ren, Gao Yong-hong, Niu Fu-ling

机构信息

First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):249-52.

PMID:17432151

Abstract

OBJECTIVE

To investigate the protective effect of geniposide, baicalin and berberine for the rat cerebral microvascular endothelial cell.

METHOD

The model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established. Both normal and model cells were treated with geniposide (1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008 micromol x mL(-1)), baicalin (0.224, 0.112, 0.056, 0.028, 0.014, 0.007, 0.003 micromol x mL(-1)) and berberine (0.192, 0.096, 0.048, 0.024, 0.012, 0.006, 0.003 micromol x mL(-1)). Cell activity was measured by methyl thiazolyl tetrazolium (MTT) test.

RESULT

After hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour, geniposide (0.128, 0.064, 0.032 micromol x mL(-1)), baicalin (0.028, 0.014, 0.007 micromol x mL(-1)) and berberine (0.024, 0.012, 0.006 micromol x microL(-1) could protect the injuried cerebral microvascular endothelial cells.

CONCLUSION

Appropriate concentration of geniposide, baicalin and berberine, which are effective components of Huanglian Jiedu decoction, could protect the injuried cerebral microvascular endothelial cells.

摘要

目的

探讨栀子苷、黄芩苷和小檗碱对大鼠脑微血管内皮细胞的保护作用。

方法

建立体外大鼠脑微血管内皮细胞缺氧复氧损伤模型。正常细胞和模型细胞分别用栀子苷（1.024、0.512、0.256、0.128、0.064、0.032、0.016、0.008 μmol·mL⁻¹）、黄芩苷（0.224、0.112、0.056、0.028、0.014、0.007、0.003 μmol·mL⁻¹）和小檗碱（0.192、0.096、0.048、0.024、0.012、0.006、0.003 μmol·mL⁻¹）处理。采用噻唑蓝（MTT）法检测细胞活性。