高效液相色谱法在不同紫外波长下测定黄连解毒汤中3种成分
[Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths].
作者信息
Li Xin-Zhong, Lei Peng, Liu Shao
机构信息
Department of Pharmacy of Xiangya Hospital, Central South University, Changsha 410008, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Oct;31(20):1686-8.
OBJECTIVE
To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.
METHOD
A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).
RESULT
This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.
CONCLUSION
The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.
目的
同时测定黄连解毒汤中3种有效成分（5种化合物），即小檗碱、巴马汀、药根碱、黄芩苷和栀子苷。
方法
采用高效液相色谱法，以3种不同紫外波长检测，小檗碱、巴马汀和药根碱检测波长为345nm，黄芩苷检测波长为280nm，栀子苷检测波长为238nm。采用Diamonsil C18（4.6mm×250mm，5μm）色谱柱，以水（A）、甲醇（B）和0.05%磷酸溶液（C）的混合液为流动相进行梯度洗脱。柱温为35℃，流速为1mL·min⁻¹。
结果
该方法可快速、准确地测定黄连解毒汤中的5种化合物。
结论
该质量控制方法对黄连解毒汤来说简便、合理且可靠。
Zotero 插件