Zhang Zhan-jun, Wang Li-ya, Wang Zhong, Li Peng-tao, Wang Yong-yan
Institute of Traditional Chinese Medicine Protection and Utilization in Beijing Normal University, Beijing 100875, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(11):907-10.
To elucidate the therapeutic effect and the influence on PI3K-Akt-PKB-BAD-CREB-PCREB pathway in focal cerebral ischemia rat responses before and after treatment with baicalin and jasminoidin given alone or in combination.
Rat model of ischemia reperfusion was established with thread. Generally accepted methods were used, including TTC staining, behavior test, as well as micro and ultra microscopy which can dynamically and accurately monitor pathological and physiological changes after cerebral ischemia on earlier period, to evaluate the brain injury induced by ischemia and the attenuations by the drugs. The difference of PI3K-Akt-PKB-BAD-CREB-PCREB expression was detected by western-blot technology.
The combination of baicalin and jasminoidin composition can be potential neuroprotective agent. TTC staining technology combined with behavior grade and ultrmicro-structure observation on brain tissue is effective method to evaluate protective agent, which is related to signal transduction PI3K-Akt-PKB-BAD-CREB-PCREB pathway. The results provide benofical basis for revealing the complex of therapeutic mechanism of traditional Chinese medicine Qingkai Ling (QKL).
阐明黄芩苷与栀子苷单独或联合给药对局灶性脑缺血大鼠治疗前后的治疗效果及对PI3K-Akt-PKB-BAD-CREB-PCREB通路的影响。
采用线栓法建立缺血再灌注大鼠模型。使用公认的方法，包括TTC染色、行为测试以及可在早期动态准确监测脑缺血后病理和生理变化的显微镜和超显微镜检查，以评估缺血诱导的脑损伤及药物的减轻作用。通过蛋白质免疫印迹技术检测PI3K-Akt-PKB-BAD-CREB-PCREB表达的差异。
黄芩苷与栀子苷组合物可能是潜在的神经保护剂。TTC染色技术结合行为评分和脑组织超微结构观察是评估保护剂的有效方法，其与信号转导PI3K-Akt-PKB-BAD-CREB-PCREB通路有关。结果为揭示中药清开灵（QKL）治疗机制的复杂性提供了有益依据。
