Hamann H, Jude R, Sieme H, Mertens U, Töpfer-Petersen E, Distl O, Leeb T
Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Anim Genet. 2007 Jun;38(3):259-64. doi: 10.1111/j.1365-2052.2007.01594.x. Epub 2007 Apr 13.
Fertility of stallions is of high economic importance, especially for large breeding organisations and studs. Breeding schemes with respect to fertility traits and selection of stallions at an early stage may be improved by including molecular genetic markers associated with traits. The genes coding for equine cysteine-rich secretory proteins (CRISPs) are promising candidate genes because previous studies have shown that CRISPs play a role in the fertilising ability of male animals. We have previously characterised the three equine CRISP genes and identified a non-synonymous polymorphism in the CRISP1 gene. In this study, we report one non-synonymous polymorphism in the CRISP2 gene and four non-synonymous polymorphisms in the CRISP3 gene. All six CRISP polymorphisms were genotyped in 107 Hanoverian breeding stallions. Insemination records of stallions were used to analyse the association between CRISP polymorphisms and fertility traits. Three statistical models were used to evaluate the influence of single mutations, genotypes and haplotypes of the polymorphisms. The CRISP3 AJ459965:c.+622G>A SNP leading to the amino acid substitution E208K was significantly associated with the fertility of stallions. Stallions heterozygous for the CRISP3 c.+622G>A SNP had lower fertility than homozygous stallions (P = 0.0234). The pregnancy rate per cycle in these stallions was estimated to be approximately 7% lower than in stallions homozygous at this position.
种公马的繁殖力具有很高的经济重要性，特别是对于大型育种组织和种马场而言。通过纳入与性状相关的分子遗传标记，关于繁殖力性状的育种方案以及早期种公马的选择可能会得到改善。编码马富含半胱氨酸分泌蛋白（CRISPs）的基因是很有前景的候选基因，因为先前的研究表明CRISPs在雄性动物的受精能力中发挥作用。我们之前已经对三个马CRISP基因进行了特征分析，并在CRISP1基因中鉴定出一个非同义多态性。在本研究中，我们报告了CRISP2基因中的一个非同义多态性和CRISP3基因中的四个非同义多态性。对107匹汉诺威种公马的所有六个CRISP多态性进行了基因分型。利用种公马的授精记录来分析CRISP多态性与繁殖力性状之间的关联。使用三种统计模型来评估多态性的单个突变、基因型和单倍型的影响。导致氨基酸替换E208K的CRISP3 AJ459965:c.+622G>A SNP与种公马的繁殖力显著相关。CRISP3 c.+622G>A SNP的杂合种公马的繁殖力低于纯合种公马（P = 0.0234）。据估计，这些种公马每个周期的妊娠率比该位置纯合的种公马低约7%。
