Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Department of Internal Medicine, Institute of Health Sciences and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju 660-702, Republic of Korea.
J Anal Methods Chem. 2014;2014:418690. doi: 10.1155/2014/418690. Epub 2014 Mar 3.
The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r (2)) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1-102.8% and 85.2-98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.
朝鲜刺叶麻 Euphorbia supina 是一种杂草，在韩国民间医学中被用于治疗多种疾病。本研究采用高效液相色谱-串联质谱法（HPLC-MS/MS）对该植物中的 9 种多酚类化合物进行了鉴定，并与文献数据进行了比较。采用结构相关的外标校准曲线对各成分进行了验证，并首次采用该验证方法进行了定量分析。校准曲线的相关系数（r (2)）均>0.9907。该方法的检测限和定量限分别为>0.028 mg/L 和 0.094 mg/L。50 mg/L 和 100 mg/L 时的回收率分别为 76.1-102.8%和 85.2-98.6%。鉴定出的多酚总量为 3352.9 ± 2.8 mg/kg 鲜重。槲皮素和山柰酚衍生物占总多酚的 84.8%。采用 1,1-二苯基-2-苦基肼基（DPPH）自由基清除活性和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）阳离子自由基清除活性评估了黄酮类化合物的抗氧化活性，还原力呈剂量依赖性增加。当多酚混合物浓度>250 mg/L 时，细胞活力被有效抑制。
