Pinel Benoit, Audo Grégoire, Mallet Sabine, Lavault Marie, De La Poype François, Séraphin Denis, Richomme Pascal
Laboratoire SONAS EA 921, UFR des Sciences Pharmaceutiques et Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
J Chromatogr A. 2007 Jun 1;1151(1-2):14-9. doi: 10.1016/j.chroma.2007.02.115. Epub 2007 Mar 16.
Plants of the Asteraceae family are known as a source of sesquiterpene lactones with interesting biological activities. The purification of several xanthanolides (xanthathin, 4-epi-xanthanol and 4-epi-isoxanthanol) was realized in one step, directly from the crude chloroformic extract of the leaves of X. macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition chromatography), using a FCPC 5L (Fast centrifugal partition chromatograph, Kromaton Technologies, Angers, France). Recovery, purity of xanthathin and solvent consumption were improved with the liquid/liquid chromatography compared to solid/liquid chromatography.
菊科植物是具有有趣生物活性的倍半萜内酯的来源。通过硅胶柱色谱法，首次使用FCPC 5L（快速离心分配色谱仪，法国昂热的克劳马顿科技公司），通过液/液色谱法（逆流色谱/离心分配色谱法）从大果黄檀叶片的粗氯仿提取物中一步实现了几种呫吨酮内酯（呫吨黄质、4-表呫吨醇和4-表异呫吨醇）的纯化。与固/液色谱法相比，液/液色谱法提高了呫吨黄质的回收率、纯度并减少了溶剂消耗。
