Funane Kazumi, Terasawa Kazue, Mizuno Yasuko, Ono Hiroshi, Miyagi Takeshi, Gibu Shigehachi, Tokashiki Tadaaki, Kawabata Yasuyuki, Kim Young-Min, Kimura Atsuo, Kobayashi Mikihiko
National Food Research Institute, 2-1-12 Kannondai, Tsukuba 305-8642, Japan.
J Biotechnol. 2007 Jun 15;130(2):188-92. doi: 10.1016/j.jbiotec.2007.03.009. Epub 2007 Mar 12.
Cyclodextrans (CIs) are cyclic isomaltooligosaccharides and only CI-7, CI-8, and CI-9 were known. CI-7, CI-8, and CI-9, consisting of seven, eight, and nine glucoses, respectively, bound by alpha-(1-->6) linkages, are known to be produced by T-3040 strain of Bacillus circulans. However, we have found, using 13C NMR and mass spectrometry, that this strain also produces CI-10, CI-11 and CI-12. These large CIs are very soluble in water and inhibit the glucan synthesis of glucansucrases to the same degree as do the smaller CIs. The CIs were thought to be poor at forming inclusion complexes with chemical compounds, due to their flexible alpha-(1-->6)-glucosidic structure. Among these six CIs, CI-10 was much better at forming an inclusion complex, and it ability to do so was as good as cyclodextrins, as determined by its ability to stabilize the color of Victoria blue B. Therefore, CI-10 may be the most commercially useful CI.
环糊精（CIs）是环状异麦芽低聚糖，之前只知道CI - 7、CI - 8和CI - 9。CI - 7、CI - 8和CI - 9分别由七个、八个和九个葡萄糖通过α-(1→6)键连接而成，已知是由环状芽孢杆菌T - 3040菌株产生的。然而，我们通过13C核磁共振和质谱分析发现，该菌株还能产生CI - 10、CI - 11和CI - 12。这些较大的环糊精在水中的溶解度非常高，并且对葡聚糖蔗糖酶葡聚糖合成的抑制程度与较小的环糊精相同。由于其具有灵活的α-(1→6)-糖苷结构，人们认为环糊精与化合物形成包合物的能力较差。在这六种环糊精中，CI - 10形成包合物的能力要强得多，通过其稳定维多利亚蓝B颜色的能力测定，其形成包合物的能力与环糊精相当。因此，CI - 10可能是最具商业用途的环糊精。
