Cole E S
Department of Biology, University of Iowa, Iowa City 52242.
Dev Biol. 1991 Dec;148(2):403-19. doi: 10.1016/0012-1606(91)90260-a.
The cortical pattern mutant broadened cortical domains (bcd) in Tetrahymena thermophila is unable to complete the nuclear events associated with conjugation. bcd x bcd pairs become arrested at the "nuclear exchange" configuration. Genetic analysis reveals that the bcd conjugal block is 100% penetrant, under macronuclear control, and rescueable (a) by outcrossing to a wild-type partner, (b) by administration of a hyperosmotic shock 5 hr after cells are mixed for mating, or (c) by cytoplasmic transfusion from a wild-type donor. Cytological analysis reveals that the conjugal block is primarily the result of failure in pronuclear fusion (karyogamy). bcd pairs also exhibit reduced nuclear exchange efficiency and a failure of macronuclear anlagen formation. The hypothesis is proposed that the bcd+ gene codes for a microtubule-based organelle "motor" similar to kinesin.
嗜热四膜虫中皮层模式突变体“皮层域扩展（bcd）”无法完成与接合相关的核事件。bcd×bcd配对在“核交换”构型处停滞。遗传分析表明，bcd接合阻断具有100%的外显率，受大核控制，并且可通过以下方式挽救：（a）与野生型伴侣杂交；（b）在细胞混合交配5小时后施加高渗休克；或（c）从野生型供体进行细胞质输注。细胞学分析表明，接合阻断主要是原核融合（核配）失败的结果。bcd配对还表现出核交换效率降低以及大核原基形成失败。有人提出假说，即bcd +基因编码一种类似于驱动蛋白的基于微管的细胞器“马达”。
